Recombinant Viral Yellow Fever Virus NS1 Protein, CF

R&D Systems | Catalog # 9776-YF

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Viral Yellow Fever Virus NS1 Protein (9776-YF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P03314
View all Yellow Fever Virus NS1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived viral Yellow Fever Virus NS1 protein
Gln780-His1134, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln780 predicted and inferred from enzymatic pyroglutamate treatment revealing Gly781. Cys782 observed.

Predicted Molecular Mass

41 kDa

SDS-PAGE

42-59 kDa, reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Viral Yellow Fever Virus NS1 Protein, CF

The Innovator Series.

The Innovator Series.

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Formulation, Preparation, and Storage

9776-YF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Yellow Fever Virus NS1

Yellow fever virus (YFV) non-structural protein 1 (NS1) is produced by yellow fever virus (YFV), a mosquito-borne flavivirus that is responsible for numerous epidemics of yellow fever disease with high fatality rates (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane‑associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral YFV NS1 contains 352 amino acids (afa) and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References

  1. Beasley, D.W. et al. (2015) Antiviral. Res. 115:48.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.

Alternate Names

YFV NS1, YFV-NS1, YFV_NS1

Entrez Gene IDs

1502173 (Viral)

Gene Symbol

POLY

UniProt

P03314

Additional Yellow Fever Virus NS1 Products

Product Documents for Recombinant Viral Yellow Fever Virus NS1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Viral Yellow Fever Virus NS1 Protein, CF

For research use only

Related Research Areas

Infectious Diseases

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