Recombinant Human Regucalcin His Protein

Name: Recombinant Human Regucalcin His Protein
Brand: Novus Biologicals
SKU: NBP1-72431
Price: 929 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-72431

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Regucalcin Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-299 of Human Regucalcin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSIKIECVL PENCRCGESP VWEEVSNSLL FVDIPAKKVC RWDSFTKQVQ RVTMDAPVSS VALRQSGGYV ATIGTKFCAL NWKEQSAVVL ATVDNDKKNN RFNDGKVDPA GRYFAGTMAE ETAPAVLERH QGALYSLFPD HHVKKYFDQV DISNGLDWSL DHKIFYYIDS LSYSVDAFDY DLQTGQISNR RSVYKLEKEE QIPDGMCIDA EGKLWVACYN GGRVIRLDPV TGKRLQTVKL PVDKTTSCCF GGKNYSEMYV TCARDGMDPE GLLRQPEAGG IFKITGLGVK GIAPYSYAG

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

35.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Regucalcin His Protein

SDS-PAGE: Recombinant Human Regucalcin His Protein [NBP1-72431]

SDS-Page: Recombinant Human Regucalcin Protein [NBP1-72431] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP1-72431

Formulation	20 mM Tris-HCl buffer (pH8.0), 2M UREA, 20% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Regucalcin

RGN (Regucalcin) belongs to the SMP-30/CGR1 family. This protein is Ca (2+)-binding protein that does not contain EF-hand motif of Ca (2+)-binding domain. RGN plays a pivotal role in the keep of intracellular Ca2+ homeostasis due to activating Ca2+ pump enzymes in the plasma membrane (basolateral membrane), microsomes (endoplasmic reticulum) and mitochondria of many cells. Also, it plays a multifunctional role in the regulation of cell functions in liver, kidney cortex, heart and brain and a suppressor protein for cell signaling systems in many cell types.

Alternate Names

EC 3.1.1.17, gluconolactonase, GNL, RCsenescence marker protein-30, regucalcin (senescence marker protein-30), Senescence marker protein 30, SMP-30, SMP30regucalcin

Gene Symbol

RGN

Additional Regucalcin Products

Product Documents for Recombinant Human Regucalcin His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Regucalcin His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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