RFP Antibody (HL2028) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25634

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2028 expressed in HEK293

Format

Azide and BSA Free
View all RFP Primary Antibodies »

Product Specifications

Immunogen

Full length RFP recombinant protein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFP Antibody (HL2028) - Azide and BSA Free

RFP Antibody (HL2028) - Azide and BSA Free

Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] -

Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted antibodies as follows: Upper image: RFP antibody [HL2028], NBP3-25634 (1:5000) Middle image: mCherry-tagged protein antibody, NBP2-43720 Lower image: FP-tagged protein antibody, The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RFP Antibody (HL2028) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] -

Immunocytochemistry/Immunofluorescence: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - RFP-tag antibody detects RFP tagged protein by immunofluorescent analysis. Sample: RFP tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP tagged protein stained by RFP-tag antibody (NBP3-25634) diluted at 500. Red: RFP tagged protein is expressed in the transfected cells. Blue: Fluoroshield with DAPI.

Applications for RFP Antibody (HL2028) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFP

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Alternate Names

DSRED, GFP-like chromoprotein, Red Fluorescent Protein, RFP

Additional RFP Products

Product Documents for RFP Antibody (HL2028) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RFP Antibody (HL2028) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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