RFP Antibody

Novus Biologicals | Catalog # NBP2-19302

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RFP Primary Antibodies »

Product Specifications

Immunogen

Full length RFP recombinant protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFP Antibody

Immunoprecipitation: RFP Antibody [NBP2-19302]

Immunoprecipitation: RFP Antibody [NBP2-19302]

Immunoprecipitation: RFP Antibody [NBP2-19302] - RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody. (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detecteddiluted at 1:10000.
RFP Antibody

Western Blot: RFP Antibody [NBP2-19302] -

Western Blot: RFP Antibody [NBP2-19302] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (NBP2-19302) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RFP Antibody

Immunocytochemistry/ Immunofluorescence: RFP Antibody [NBP2-19302] -

RFP antibody detects RFP protein at by immunofluorescent analysis.
Sample: RFP tagged protein transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RFP protein stained by RFP antibody (NBP2-19302) diluted at 1:5000.
Red: RFP is expressed in the RFP-fusion protein tranfected cell.
Blue: Hoechst 33342 staining.
The cell of merger panel with no RFP expressed as a negative control.
RFP Antibody

Western Blot: RFP Antibody [NBP2-19302] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (NBP2-19302) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RFP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:5000

Immunoprecipitation

1:10000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RFP

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Alternate Names

DSRED, GFP-like chromoprotein, Red Fluorescent Protein, RFP

Additional RFP Products

Product Documents for RFP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RFP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for RFP Antibody

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  • Q: Is this antibody (NBP2-19302) also working against tdTomato? I would like to use antibody for Immunohistochemistry or Immunofluorescence.

    A:

    NBP2-19302 has never been tested for cross-reactivity to dsRed derivatives. NBP2-19302 will most likely cross-react though as TdTomato and dsRed are over 90% similar. As you need an antibody for IHC, I do recommend NBP2-19302, as though it has never been tested in tissues, it does work well on cells. You would be eligible for our Innovator's Reward program.

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