RFPL1 Antibody (OTI1D3)

Novus Biologicals | Catalog # NBP2-02835

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D3
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Product Specifications

Immunogen

Full length human recombinant protein of human RFPL1 (NP_066306) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

35.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFPL1 Antibody (OTI1D3)

Western Blot: RFPL1 Antibody (OTI1D3) [NBP2-02835]

Western Blot: RFPL1 Antibody (OTI1D3) [NBP2-02835]

Western Blot: RFPL1 Antibody (1D3) [NBP2-02835] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFPL1.
Immunocytochemistry/Immunofluorescence: RFPL1 Antibody (1D3) [NBP2-02835] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RFPL1.
Flow Cytometry: RFPL1 Antibody (OTI1D3) [NBP2-02835]

Flow Cytometry: RFPL1 Antibody (OTI1D3) [NBP2-02835]

Flow Cytometry: RFPL1 Antibody (1D3) [NBP2-02835] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RFPL1 antibody, and then analyzed by flow cytometry.

Applications for RFPL1 Antibody (OTI1D3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.72 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RFPL1

RFPL1 is involved in ubiquitination and zinc ion binding.

Alternate Names

MGC132428, ret finger protein-like 1, RFPL1L, RNF78RING finger protein 78

Entrez Gene IDs

5988 (Human)

Gene Symbol

RFPL1

Additional RFPL1 Products

Product Documents for RFPL1 Antibody (OTI1D3)

Certificate of Analysis

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Product Specific Notices for RFPL1 Antibody (OTI1D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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