Rif1 Antibody

Novus Biologicals | Catalog # NBP2-26129

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Rif1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RNLQSRWRSPSHEN corresponding to C-Terminus according to NP_060621.3, NP_001171134.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rif1 Antibody

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-26129]

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-26129]

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-26129] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-26129]

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-26129]

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-26129] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Rif1 Antibody

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-26129] -

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-26129] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Rif1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rif1

Rif1 (Rap1-interacting factor homolog) is a protein whose primary function is in the DNA-damage response, RIF1 forms foci that colocalize with other DNA damage response factors, after induction of double-strand breaks. Rif1 localizes to double strand breaks in an ATM (ataxia telangiectasia mutated)- and 53BP1-dependent manner and functions in the intra-S-phase checkpoint that serves to slow down DNA synthesis when DNA damage has occurred.

Alternate Names

DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Entrez Gene IDs

55183 (Human); 51869 (Mouse)

Gene Symbol

RIF1

Additional Rif1 Products

Product Documents for Rif1 Antibody

Certificate of Analysis

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Product Specific Notices for Rif1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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