rM. Thermoautotrophicus TDG with Buffer

Discontinued Product

4070-500-EB has been discontinued.
View all TDG products.
Product Details
Citations (4)

rM. Thermoautotrophicus TDG with Buffer Summary

Kit containing rM. Thermoautotrophicus TDG and a buffer.

Product Description

TDG is a thermostable thymine DNA glycosylase from Methanobacterium thermoautotrophicum. The optimal temperature for the enzyme is 65°C. The enzyme lacks significant AP lyase or endonuclease activity. TDG works effectively in heteroduplex analysis to detect C to T transitions.

TDG enzyme recognizes T/G mismatches in duplex DNA and cleaves the strand with the T. The opposite strand is not cleaved. The enzyme also recognizes G/G mismatches if at least one nearest neighbor is an A or T and nicks one strand or the other. The enzyme exhibits poor AP lyase activity. One Unit is the amount of enzyme required to cleave 1 pmole of an oligonucleotide duplex containing a T/G mismatch in 1 hour at 65 °C. Only the strand containing the T is cleaved.

Assay Conditions and Analysis

4 pmoles of T/G mismatch oligonucleotide set with the T oligo end-labeled, 1X REC Buffer 4 (10 mM HEPES-KOH (pH 7.4), 100 mM KCl, and 10 mM EDTA), and serial dilutions of enzyme in a 20 μL reaction volume are incubated for 1 hour at 65 °C. To complete cleavage of a basic site, fresh 1N NaOH is added to final concentration of 166 mM then heated for 15 minutes at 95 °C. For analysis, 24 μL of 2X Loading Buffer (20 mM EDTA, 95% formamide, and 0.13% bromophenol blue) are added, and the samples heated at 95 °C for 10 min then fast cooled to 2-8 °C. The cleavage products are resolved by 20% denaturing polyacrylamide gel electrophoresis, and percent cleavage quantified.

Kit Contents

• 10X REC™ Reaction Buffer 4
• Thermostable TDG Enzyme


Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.


For research use only. Not for diagnostic use.

Product Datasheets

You must select a language.


Citations for rM. Thermoautotrophicus TDG with Buffer

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Label-free and amplified electrogenerated chemiluminescence biosensing for the detection of thymine DNA glycosylase activity using DNA-functionalized gold nanoparticles triggered hybridization chain reaction
    Authors: W Bai, Y Wei, Y Zhang, L Bao, Y Li
    Anal. Chim. Acta, 2019;1061(0):101-109.  2019
  2. Controllable Autocatalytic Cleavage-Mediated Fluorescence Recovery for Homogeneous Sensing of Alkyladenine DNA Glycosylase from Human Cancer Cells
    Authors: LJ Wang, ML Luo, XY Yang, XF Li, Y Wu, CY Zhang
    Theranostics, 2019;9(15):4450-4460.  2019
  3. DNA cytosine and methylcytosine deamination by APOBEC3B: enhancing methylcytosine deamination by engineering APOBEC3B.
    Authors: Fu Y, Ito F, Zhang G, Fernandez B, Yang H, Chen X
    Biochem J, 0;471(1):25-35.  0
  4. Methylcytosine and normal cytosine deamination by the foreign DNA restriction enzyme APOBEC3A.
    Authors: Carpenter M, Li M, Rathore A, Lackey L, Law E, Land A, Leonard B, Shandilya S, Bohn M, Schiffer C, Brown W, Harris R
    J Biol Chem, 0;287(41):34801-8.  0


No product specific FAQs exist for this product, however you may

View all FAQs

Reviews for rM. Thermoautotrophicus TDG with Buffer

There are currently no reviews for this product. Be the first to review rM. Thermoautotrophicus TDG with Buffer and earn rewards!

Have you used rM. Thermoautotrophicus TDG with Buffer?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review