RMR-Tre

Tocris Bioscience | Catalog # 8013

Far-red fluorogenic trehalose probe for live mycobacteria imaging
Tocris Bioscience

Key Product Details

Description

Far-red fluorogenic trehalose probe for live mycobacteria imaging

Wavelength

Excitation = 536 nm, Emission = 684 nm
View all Fluorescent Probes for Imaging Bacteria »

Product Description

RMR-Tre is a far-red fluorogenic trehalose specific probe for live mycobacteria imaging. It enables fast, no-wash, low-background fluorescence detection of live mycobacterial outer membrane. RMR-Tre contains a molecular rotor fluorophore that brightly emits signal in the far-red region upon trehalose-guided metabolic integration into the mycomembrane. It exhibited up to a 100- fold enhancement in Mycobacterium tuberculosis labeling compared to existing fluorogenic trehalose probes. Excitation and emission maxima (λ) are 549 nm and 671 nm, respectively.

Scientific Data Images for RMR-Tre

Far-red fluorogenic trehalose probe for live mycobacteria imaging

Application of RMR-Tre.

Image shows fluorescence detection of live mycobacterial outer membrane: M. smegmatis were incubated for 1 h in the presence of 100 μM of RMR-Tre, then directly imaged by fluorescence microscopy without washing. Scale bar: 10 μm.

Licensing Information

Sold under license from Central Michigan University

Optical Data

RMR-Tre Dye Spectra
Emission Color Far Red
λabs 536 nm
λem 684 nm
Closest Laser line 540 nm

Spectra Viewer

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Product Specifications for RMR-Tre

Molecular Weight

686.73

Formula

C32H38N4O11S

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

3-(6-(5-(2,2-Dicyanovinyl)thiophen-2-yl)-3,4-dihydroquinolin-1(2H)-yl)-N-(((2R,3S,4S,5R,6R)-3,4,5-trihydroxy-6-(((2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)methyl)propanamide

PubChem ID

172005912

InChI Key

SSAVIDOUSMJZDG-IVJDZZAPSA-N

SMILES

O[C@H]1[C@@H]([C@H]([C@H](O[C@@H]1CO)O[C@@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CNC(CCN3C4=C(CCC3)C=C(C5=CC=C(S5)/C=C(C#N)/C#N)C=C4)=O)O)O)O)O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.87 10

Preparing Stock Solutions for RMR-Tre

The following data is based on the product molecular weight 686.73.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 14.56 mL 72.81 mL 145.62 mL
0.5 mM 2.91 mL 14.56 mL 29.12 mL
1 mM 1.46 mL 7.28 mL 14.56 mL
5 mM 0.29 mL 1.46 mL 2.91 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for RMR-Tre

Certificate of Analysis

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Product Specific Notices for RMR-Tre

For research use only

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