RNA Imaging Probe 1c
Tocris Bioscience | Catalog # 8813
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Product Description
RNA Imaging Probe 1c is a fluorogenic RNA imaging probe. RNA Imaging Probe 1c displays high membrane permeability, strong fluorogenic responses upon binding RNA, compatibility with fluorescence lifetime imaging microscopy (FLIM), low cytotoxicity, and excellent photostability. Excitation and emission maxima (λ) are 556 nm and 608 nm, respectively; quantum yield = 0.49; extinction coefficient = 27,500 M-1cm-1. Suitable for live cell imaging.Scientific Data Images for RNA Imaging Probe 1c
Application of RNA Imaging Probe 1c on live HeLa cells.
Confocal fluorescence images of live Hela cells imaged with 20 μM methyl pyridinium indole (MPI) (green) ( lambda ex = 470 nm, lambda em = 525-555 nm) and 20 μM RNA Imaging Probe 1c (red) ( lambda ex = 550 nm, lambda em= 580-620 nm) for 30 min. Scale bar: 10 μm.
Application of RNA Imaging Probe 1c on PFA-fixed HeLa cells.
Confocal fluorescence images of PFA-fixed HeLa cells incubated with 20 μM RNA Imaging Probe 1c (red) and 1 μg/mL Hoechst 33342 (blue) for 30 min.
Application of RNA Imaging Probe 1c on HeLa cells.
Zoomed-in confocal fluorescence image of HeLa cells stained with 20 μM RNA Imaging Probe 1c for 30 min. Image shows the sub-nucleolar components fibrillar center (FC) (indicated by the white arrowhead), dense fibrillar component (DFC), and outer granular component (GC) (indicated by the white arrows). Scale bar: 3 μm.
Yeast RNA and spermine labeled with RNA Imaging Probe 1c.
Torula yeast RNA and spermine were incubated with RNA Imaging Probe 1c (10 μM). FLIM images show the formation of spherical coacervate droplets upon mixing the RNA and spermine. Image is enlarged by 1× (on the left) or by 8× (on the right). Scale bar: 10 μm.Licensing Information
Sold under license from the University of Southern California.Optical Data
| λabs | 556 nm |
| λem | 608 nm |
| Extinction Coefficient (ε) | 27500 M-1cm-1 |
| Quantum Yield (φ) | 0.49 |
| Cell Permeable | Yes |
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Product Specifications for RNA Imaging Probe 1c
Molecular Weight
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Chemical Name
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PubChem ID
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The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.24 | 20 |
Preparing Stock Solutions for RNA Imaging Probe 1c
The following data is based on the product molecular weight 362.21.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 13.80 mL | 69.02 mL | 138.04 mL |
| 1 mM | 2.76 mL | 13.80 mL | 27.61 mL |
| 2 mM | 1.38 mL | 6.90 mL | 13.80 mL |
| 10 mM | 0.28 mL | 1.38 mL | 2.76 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Kim Development of highly fluorogenic styrene probes for visualizing RNA in live cells. ACS Chem.Biol. 2023 PMID: 37200527
Product Documents for RNA Imaging Probe 1c
Certificate of Analysis
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Product Specific Notices for RNA Imaging Probe 1c
For research use only
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