Ro 31-8220 mesylate

Key Product Details

Product Description

Ro 31-8220 mesylate is a protein kinase C inhibitor, with activity at other protein kinases (IC₅₀ values are 33, 3, 8, 15 and 38 nM for PKCα, MAPKAP-K1b, MSK1, GSK3β and S6K1 respectively). Activates JNK and glycogen synthase, and inhibits MAPK and ERK2, in rat adipocytes and L6 myotubes. Also inhibits voltage-dependent Na⁺ channels in the micromolar range. Induces protein-protein interaction between mutant SMAD4^R361H and SMAD3, leading to reactivation of dormant SMAD4^R361H-mediated transcriptional activity and restoration of TGF-β-induced tumor suppression activity in SMAD4 mutant cancer cells.

Product Specifications for Ro 31-8220 mesylate

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for Ro 31-8220 mesylate

The following data is based on the product molecular weight 553.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.81 mL	9.03 mL	18.06 mL
5 mM	0.36 mL	1.81 mL	3.61 mL
10 mM	0.18 mL	0.90 mL	1.81 mL
50 mM	0.04 mL	0.18 mL	0.36 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 335 publications citing the usage of this product.

• Tang Hypomorph mutation-directed small-molecule protein-protein interaction inducers to restore mutant SMAD4-suppressed TGF-β signaling. Cell Chem.Biol. 2020 PMID: 33326750
• Standaert RO 31-8220 activates c-Jun N-terminal kinase and glycogen synthase in rat adipocytes and L6 myotubes. Comparison to actions of Ins. Endocrinology 1999 PMID: 10218965
• Lingameneni Inhibition of voltage-dependent sodium channels by Ro 31-8220, a 'specific' protein kinase C inhibitor. FEBS Lett. 2000 PMID: 10812087
• Davies Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem.J. 2000 PMID: 10998351
• Davis Potent, selective inhibitors of protein kinase C. FEBS Lett. 1989 PMID: 2532156

Product Documents for Ro 31-8220 mesylate