RPL10 Antibody (OTI6B11) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73944

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6B11

Format

Azide and BSA Free
View all RPL10 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RPL10 (NP_006004) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPL10 Antibody (OTI6B11) - Azide and BSA Free

Western Blot: RPL10 Antibody (OTI6B11)Azide and BSA Free [NBP2-73944]

Western Blot: RPL10 Antibody (OTI6B11)Azide and BSA Free [NBP2-73944]

Western Blot: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPL10.
Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)
Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)(1:150)
Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944]

Immunohistochemistry: RPL10 Antibody (OTI6B11) - Azide and BSA Free [NBP2-73944] - Analysis of Adenocarcinoma of Human colon tissue.(Heat-induced epitope retrieval by Tris-EDTA, pH8.0)

Applications for RPL10 Antibody (OTI6B11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RPL10

The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. A distant member of the MAP kinase family, designated c-Jun NH2-terminal kinase (JNK1) functions to regulate c-Jun by phosphorylation at the amino terminal serine regulatory sites, Ser 63 and Ser 73). QM has been described as a transcription factor that can function to bind DNA directly or alternatively can interact with c-Jun to inhibit transactivation of AP-1 promoter driven reporter vectors by Jun-Jun homodimers. QM is highly conserved throughout eukaryotic evolution and is apparently a member of a multi-gene family.

Alternate Names

60S ribosomal protein L10, DXS648EFLJ27072, DXS648FLJ23544, Laminin receptor homolog, NOV, Protein QM, QMDKFZp686J1851, ribosomal protein L10, Tumor suppressor QM, Wilms tumor-related protein

Gene Symbol

RPL10

Additional RPL10 Products

Product Documents for RPL10 Antibody (OTI6B11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RPL10 Antibody (OTI6B11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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