RPL10 Antibody - BSA Free

Immunohistochemistry-Paraffin: RPL10 Antibody [NBP1-84037]

Immunohistochemistry-Paraffin: RPL10 Antibody [NBP1-84037] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Western Blot: RPL10 Antibody - BSA Free [NBP1-84037]

Analysis in human cell line HeLa.

Western Blot: RPL10 Antibody - BSA Free [NBP1-84037]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Western Blot: RPL10 Antibody - BSA Free [NBP1-84037] -

The effect of Rpl24Bst mutation on translation and ribosome composition.(A) Representative polysome profiles from Apcfl/fl KrasG12D/+ or Apcfl/fl KrasG12D/+ Rpl24Bst/+ small intestinal organoid cultures, pre-treated with harringtonine for 5 min/300 s (H300) or untreated (H0). These traces were analysed for the run-off rates shown in Figure 3E. (B) Representative polysome profiles from Apcfl/+ KrasG12D/+ or Apcfl/+ KrasG12D/+ Rpl24Bst/+ colonic adenoma cultures (left) and quantification of the polysome to subpolysome ratio from these (right). Two biologically independent lines were analysed per genotype and plotted +/- standard error of the mean (SEM). Scheme above denotes the generation of adenoma cultures from distinct colonic tumours in aged VillinCreER Apcfl/+ KrasG12D/+ and VillinCreER Apcfl/+ KrasG12D/+ Rpl24Bst/+ mice. (C) Relative protein synthesis rates quantified by 35S-methionine incorporation in the colonic adenoma cultures described in (B) with n = 3. The average protein synthesis rates were plotted relative to Apcfl/+ KrasG12D/+ controls (=1) for three organoid lines per genotype +/- SEM. Significance was determined by Mann–Whitney U test. (D) 60S to 40S ratio from sucrose density gradients from lysates generated from the indicated genotypes. Data show the mean +/- SEM. Representative traces are shown in Figure 3B and Figure 4—figure supplement 2B. (E) Western blotting from protein lysates generated from three biologically independent organoid lines for each genotype. Mean values for RPL24, RPL10, RPL22, and RPS6 expression relative to beta -actin and P-eEF2 T56 relative to eEF2 are shown next to the relevant blot +/- SEM. The average of the VillinCreER Apcfl/+ KrasG12D/+ lanes in all cases has been set to 1. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/69729), licensed under a CC-BY license. Not internally tested by Novus Biologicals.