RPL13 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-57476

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPL13 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to RPL13 (ribosomal protein L13) The peptide sequence was selected from the C terminal of RPL13 (NP_000968). Peptide sequence KKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for RPL13 Antibody - BSA Free

Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: RPL13 Antibody [NBP1-57476]

Immunohistochemistry: RPL13 Antibody [NBP1-57476]

Immunohistochemistry: RPL13 Antibody [NBP1-57476] - Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasmic in alveolar mostly type I cells Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476] - Jurkat cell lysate, concentration 0.0625ug/ml.
Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml RPL13 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.
Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476] - Hela, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that RPL13 is expressed in HeLa.
Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.
Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476]

Western Blot: RPL13 Antibody [NBP1-57476] - MCF7, Antibody Dilution: 1.0 ug/ml RPL13 is strongly supported by BioGPS gene expression data to be expressed in MCF7.

Applications for RPL13 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPL13

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL13 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Transcript variants derived from alternative splicing and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Alternate Names

BBC1FLJ27454, Breast basic conserved protein 1, D16S444E, FLJ27453, MGC117342, MGC71373,60S ribosomal protein L13, OK/SW-cl.46, ribosomal protein L13

Entrez Gene IDs

6137 (Human)

Gene Symbol

RPL13

UniProt

P26373 (Human)

Additional RPL13 Products

Product Documents for RPL13 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPL13 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RPL13 Antibody - BSA Free

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Protocols

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FAQs

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