RPL18A Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP2-30708PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Antibody Competition
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Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human RPL18A.

Source: E. coli

Amino Acid Sequence: RAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-30708.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP2-30708PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: RPL18A

RPL18A, also referred to as 60S Ribosomal Protein L18A, is a component of the large 60S subunit of ribosomes. RPL18A is 176 amino acids in length and is approximately 20kDa. RPL18A has a high binding affinity for Importin 9, a nuclear transport receptor that averts aggregation of RPS7 and RPL18A in the cytoplasm. Current research on RPL18A has shown a possible link with hepatitis C, malaria and Treacher Collins syndrome. RPL18A has also been shown to interact with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D and HIST1H4E.

Alternate Names

60S ribosomal protein L18a, ribosomal protein L18a, ribosomal protein L18a-like protein

Gene Symbol

RPL18A

Additional RPL18A Products

Product Documents for RPL18A Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for RPL18A Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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