RRAS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32156

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRAS. The exact sequence is proprietary.

Localization

Cell membrane, Lipid-anchor, Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RRAS Antibody - BSA Free

Western Blot: RRAS Antibody [NBP1-32156]

Western Blot: RRAS Antibody [NBP1-32156]

Western Blot: RRAS Antibody [NBP1-32156] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RRAS antibody [N1C3] diluted at 1:500.
Immunohistochemistry-Paraffin: RRAS Antibody [NBP1-32156]

Immunohistochemistry-Paraffin: RRAS Antibody [NBP1-32156]

Immunohistochemistry-Paraffin: RRAS Antibody [NBP1-32156] - Paraffin-embedded mouse heart. RRAS antibody [N1C3] diluted at 1:500.
Western Blot: RRAS Antibody [NBP1-32156]

Western Blot: RRAS Antibody [NBP1-32156]

Western Blot: RRAS Antibody [NBP1-32156] - Sample(30 ug of whole cell lysate) A:MOLT4 B:Raji 12% SDS PAGE, antibody diluted at 1:500.
Western Blot: RRAS Antibody [NBP1-32156]

Western Blot: RRAS Antibody [NBP1-32156]

Western Blot: RRAS Antibody [NBP1-32156] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RRAS antibody [N1C3] diluted at 1:500.
Immunohistochemistry-Paraffin: RRAS Antibody [NBP1-32156]

Immunohistochemistry-Paraffin: RRAS Antibody [NBP1-32156]

Immunohistochemistry-Paraffin: RRAS Antibody [NBP1-32156] - Paraffin-embedded Hep3B xenograft, using antibody at 1:100 dilution.

Applications for RRAS Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RRAS

R-Ras is a member of the Ras superfamily of GTPases. Ras proteins function as signaling focal points that are activated by convergent signaling pathways intiated by extracellular stimuli (reviewed in Mitin et al, 2005). Activated Ras proteins in turn regulate a diversity of downstream cytoplasmic signaling cascades. Many members of the Ras superfamily are implicated in the regulation of hematopoietic cells, with roles in growth, survival, differentiation, cytokine production, chemotaxis, vesicle-trafficking, and phagocytosis. The Ras superfamily of proteins includes over 150 small GTPases (distinguished from the large, heterotrimeric GTPases, the G-proteins). It comprises six subfamilies, the Ras, Rho, Ran, Rab, Arf, and Kir/Rem/Rad subfamilies. They exhibit considerable overall amino acid identities, especially in the regions interacting with the guanine nucleotide exchange factors that catalyze their activation. Recognizes R-Ras; human R-Ras is a 218 amino acid protein.

Alternate Names

Oncogene RRAS, p23, ras-related protein R-Ras, related RAS viral (r-ras) oncogene homolog

Gene Symbol

RRAS

Additional RRAS Products

Product Documents for RRAS Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RRAS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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