Ryanodine Receptor 2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-95111

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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Ryanodine Receptor 2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 4868-4967 of human Ryanodine Receptor 2 (NP_001026.2). EHTGQESYVWKMYQERC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ryanodine Receptor 2 Antibody - Azide and BSA Free

Ryanodine Receptor 2 Antibody - Azide and BSA Free

Western Blot: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] -

Western Blot: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] - Western blot analysis of lysates from Mouse heart, using Ryanodine Receptor 2 Rabbit pAb at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Ryanodine Receptor 2 Antibody - Azide and BSA Free

Immunohistochemistry: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] -

Immunohistochemistry: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] - Immunohistochemistry analysis of paraffin-embedded Human heart tissue using Ryanodine Receptor 2 Rabbit pAb at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Ryanodine Receptor 2 Antibody - Azide and BSA Free

Immunohistochemistry: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] -

Immunohistochemistry: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using Ryanodine Receptor 2 Rabbit pAb at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Ryanodine Receptor 2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] -

Immunocytochemistry/ Immunofluorescence: Ryanodine Receptor 2 Antibody - Azide and BSA Free [Ryanodine Receptor 2] - Immunofluorescence analysis of paraffin-embedded mouse heart using Ryanodine Receptor 2 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Ryanodine Receptor 2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ryanodine Receptor 2

Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The alpha1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR alpha1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR alpha1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.

Long Name

Ryanodine receptor 2

Alternate Names

hRYR-2, RYR-2, RyR2

Gene Symbol

RYR2

Additional Ryanodine Receptor 2 Products

Product Documents for Ryanodine Receptor 2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Ryanodine Receptor 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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