Recombinant Mouse S100A8 His Protein
Novus Biologicals | Catalog # NBP1-72331
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Key Product Details
Source
E. coli
Tag
His
Applications
SDS-PAGE
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Product Specifications
Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-89 of Mouse S100A8
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSELEKALS NLIDVYHNYS NIQGNHHALY KNDFKKMVTT ECPQFVQNIN IENLFRELDI NSDNAINFEE FLAMVIKVGV ASHKDSHKE
Purity
>95%, by SDS-PAGE
Predicted Molecular Mass
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Mouse S100A8 His Protein
SDS-PAGE: Recombinant Mouse S100A8 His Protein [NBP1-72331]
SDS-Page: Recombinant Mouse S100A8 Protein [NBP1-72331]Formulation, Preparation, and Storage
NBP1-72331
| Formulation | 20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1 M NaCl, 1 mM DTT |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: S100A8
Long Name
S100 Calcium Binding Protein A8
Alternate Names
Calgranulin A, CFAG, MRP8
Gene Symbol
S100A8
Additional S100A8 Products
Product Documents for Recombinant Mouse S100A8 His Protein
Product Specific Notices for Recombinant Mouse S100A8 His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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FAQs for Recombinant Mouse S100A8 His Protein
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Q: Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
A: Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link. -
Q: We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
A: Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
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