SARS-CoV-2 NSP9 Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # FAB10995AFP594

R&D Systems

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1044209
View all NSP9 Primary Antibodies »

Product Specifications

Specificity

Detects SARS-CoV2 NSP9 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for SARS-CoV-2 NSP9 Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

ELISA

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NSP9

Non-structural protein 9 (NSP9) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP9 is a small 113 amino acid protein that forms a biologically active homodimer where each monomer consists of a beta barrel and C-terminal helical domain motif that promotes obligate dimerization (2,3). NSP9 is capable of binding nucleic acids in a nonsequence-specific manner with a preference of a single stranded RNA (4,5) although disruption of the dimeric interface appears to impact RNA binding (6). The NSP9 sequence is conserved across coronaviruses (3). NSP9 was shown to interact with other viral NSP proteins including NSP7, NSP8, and NSP12 (5,7,8). In addition, NSP9 has been shown to bind host cell proteins including DEAD-box RNA helicase 5 (DDX5), the ubiquitin E3 ubiquitin ligase MIB1, and elongation factor eIF4H in SARS-CoV2 and related viruses (9,10). The interactions of NSP9 with these host cell proteins promote viral replication (9,10) supporting the conclusion that NSP9 is important for virulence (2,3).

References

  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59.
  2. Miknis, Z.J. et al. (2009) J. Virol. 83:3007. 
  3. Littler, D.R. et al. (2020) iScience 23:101258
  4. Egloff, M.P. et al. (2004) Proc. Nat. Acad. Sci. U.S.A. 101:3792.
  5. Sutton, G. et. al. (2004) Structure 12:341.
  6. Hu, T. et al. (2017) Protein Sci. 26:1037.
  7. von Brunn, A. et al. (2007) PLoS One 2:e459.
  8. Chen, J. et al. (2017) Front. Microbiol. 8:853.
  9. Zhao, S. et al. (2015) Virus Res. 195:217.
  10. Gordon, D.E. et al. (2020) Nature 583:459.

Long Name

Non-Structural Protein 9

Alternate Names

Non-structural protein 9, ORF1a polyprotein, ORF1ab polyprotein, p12

UniProt

YP_009725305.1

Additional NSP9 Products

Product Documents for SARS-CoV-2 NSP9 Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for SARS-CoV-2 NSP9 Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Infectious Diseases

Customer Reviews

There are currently no reviews for this product. Be the first to review SARS-CoV-2 NSP9 Alexa Fluor™ Plus 594‑conjugated Antibody and earn rewards!

Have you used SARS-CoV-2 NSP9 Alexa Fluor™ Plus 594‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies