SASH1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-26651
![Immunohistochemistry: SASH1 Antibody [NBP1-26651]](https://resources.rndsystems.com/images/products/SASH1-Antibody-Immunohistochemistry-NBP1-26651-img0004.jpg "Immunohistochemistry: SASH1 Antibody [NBP1-26651]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot (Negative)
Cited:
Immunohistochemistry-Paraffin, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human SAM and SH3 domain containing 1 using the numbering given in entry NP_056093.3 (GeneID 23328).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SASH1 Antibody - BSA Free
Immunohistochemistry: SASH1 Antibody [NBP1-26651]
Immunohistochemistry: SASH1 Antibody [NBP1-26651] - Sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and lightchain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.![Immunoprecipitation: SASH1 Antibody [NBP1-26651]](https://resources.rndsystems.com/images/products/SASH1-Antibody-Immunoprecipitation-NBP1-26651-img0002.jpg "Immunoprecipitation: SASH1 Antibody [NBP1-26651]")
Immunoprecipitation: SASH1 Antibody [NBP1-26651]
Immunoprecipitation: SASH1 Antibody [NBP1-26651] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 3 mcg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti- SASH1 antibody NBP1-26650, which recognizes an upstream epitope.Applications for SASH1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250-1:1000
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
3-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SASH1
Alternate Names
dJ323M4, dJ323M4.1, KIAA0790RP3-323M4.1, PEPE1, Proline-glutamate repeat-containing protein, SAM and SH3 domain containing 1,2500002E12Rik, SAM and SH3 domain-containing protein 1, SH3D6A
Entrez Gene IDs
23328 (Human)
Gene Symbol
SASH1
UniProt
Additional SASH1 Products
Product Documents for SASH1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SASH1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SASH1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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