SCFD1 Antibody (OTI5G4) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-74004
![Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Western-Blot-NBP2-74004-img0002.jpg "Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]")
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI5G4
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human SCFD1(NP_057190) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SCFD1 Antibody (OTI5G4) - Azide and BSA Free
Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]
Western Blot: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCFD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCFD1.![Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74004-img0008.jpg "Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]")
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Staining of paraffin-embedded Human Kidney tissue using anti-SCFD1 mouse monoclonal antibody.![Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Western-Blot-NBP2-74004-img0001.jpg "Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]")
Western Blot: SCFD1 Antibody (OTI5G4)Azide and BSA Free [NBP2-74004]
Western Blot: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-SCFD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).![Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74004-img0003.jpg "Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]")
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SCFD1 mouse monoclonal antibody.![Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74004-img0004.jpg "Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]")
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SCFD1 mouse monoclonal antibody.![Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74004-img0005.jpg "Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]")
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SCFD1 mouse monoclonal antibody.![Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74004-img0006.jpg "Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]")
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SCFD1 mouse monoclonal antibody.![Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74004-img0007.jpg "Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]")
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SCFD1 mouse monoclonal antibody.![Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]](https://resources.rndsystems.com/images/products/SCFD1-Antibody-OTI5G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74004-img0009.jpg "Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]")
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004]
Immunohistochemistry: SCFD1 Antibody (OTI5G4) - Azide and BSA Free [NBP2-74004] - Staining of paraffin-embedded Human pancreas tissue using anti-SCFD1 mouse monoclonal antibody.Applications for SCFD1 Antibody (OTI5G4) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: SCFD1
Alternate Names
C14orf163, KIAA0917chromosome 14 open reading frame 163, sec1 family domain containing 1, sec1 family domain-containing protein 1, SLY1, SLY1 homolog, Sly1p, STXBP1L2RA410, Syntaxin-binding protein 1-like 2, vesicle transport-related protein
Gene Symbol
SCFD1
Additional SCFD1 Products
Product Documents for SCFD1 Antibody (OTI5G4) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SCFD1 Antibody (OTI5G4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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