sCy5DL-amide

Tocris Bioscience | Catalog # 7835

FDAA for super-resolution microscopy of bacteria
Tocris Bioscience

Key Product Details

Description

FDAA for super-resolution microscopy of bacteria

Wavelength

Excitation = 646 nm, Emission = 666 nm
View all Fluorescent Probes for Imaging Bacteria »

Product Description

sCy5DL-amide is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into Gram-positive B.subtilis. sCy5DL-amide labeled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/666 nm.

Scientific Data Images for sCy5DL-amide

Bacillus subtilis cells stained with sCy5DA-amide

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA-amide

Optical Data

sCy5DL-amide Dye Spectra
Emission Color Red
λabs 646 nm
λem 666 nm
Extinction Coefficient (ε) 209,200 M-1cm-1
Cell Permeable Yes
Application STORM

Product Specifications for sCy5DL-amide

Molecular Weight

769.97

Formula

C38H51N5O8S2

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-((1E,3E)-5-((E)-1-(6-(((R)-6-Amino-5-ammonio-6-oxohexyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)penta-1,3-dien-1-yl)-1,3,3-trimethyl-3H-indol-1-ium-5-sulfonate

InChI Key

KASKHZOOXRBMET-SSEXGKCCSA-N

SMILES

CC1(C2=C(N(/C1=C/C=C/C=C/C3=[N+](C)C4=C(C=C(C=C4)S(=O)([O-])=O)C3(C)C)CCCCCC(NCCCC[C@@H]([NH3+])C(N)=O)=O)C=CC(S(=O)([O-])=O)=C2)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.7 10

Preparing Stock Solutions for sCy5DL-amide

The following data is based on the product molecular weight 769.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 12.99 mL 64.94 mL 129.88 mL
0.5 mM 2.60 mL 12.99 mL 25.98 mL
1 mM 1.30 mL 6.49 mL 12.99 mL
5 mM 0.26 mL 1.30 mL 2.60 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for sCy5DL-amide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for sCy5DL-amide

For research use only

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