Serpin F1/PEDF Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-19767
Loading...
Key Product Details
Species Reactivity
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Serpin F1/PEDF. The exact sequence is proprietary.
Reactivity Notes
Rat (83%), Dog (87%), Pig (87%), Sheep (86%), Bovine (86%), Guinea pig (83%).
Localization
Secreted; Melanosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Serpin F1/PEDF Antibody - BSA Free
Western Blot: Serpin F1/PEDF Antibody [NBP2-19767] -
HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PEDF antibody [N1C2] (NBP2-19767) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry: Serpin F1/PEDF Antibody - BSA Free [NBP2-19767] -
PEDF in the spiral ganglion following furosemide injection. (A–F) PEDF protein staining of guinea pig spiral ganglion following IV furosemide injection. (A) In the control was PEDF localized in the cytoplasm of the spiral ganglion type I neurons. (B–E) The staining intensity after furosemide at the different intervals. (F) Negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35392272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunohistochemistry: Serpin F1/PEDF Antibody - BSA Free [NBP2-19767] -
PEDF in the spiral ganglion following furosemide injection. (A–F) PEDF protein staining of guinea pig spiral ganglion following IV furosemide injection. (A) In the control was PEDF localized in the cytoplasm of the spiral ganglion type I neurons. (B–E) The staining intensity after furosemide at the different intervals. (F) Negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35392272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunohistochemistry: Serpin F1/PEDF Antibody - BSA Free [NBP2-19767] -
PEDF in the lateral cochlear wall. PEDF protein localization in the lateral cochlear wall. Stria vascularis (SV); Spiral prominence (SP); Marginal cell (MC); Basal cell (BC); type I fibrocytes (*); type II fibrocytes (**). (A) PEDF was localized to the spiral ligament, the stria vascularis and the spiral prominence. In the spiral ligament were type I and II fibrocytes PEDF positive. (B) Inset of higher magnification of marked area in (A). The distribution of PEDF in sub-cellular level of stria vascularis and spiral ligament. (C) Negative control of the immunohistochemistry. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35392272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunohistochemistry: Serpin F1/PEDF Antibody - BSA Free [NBP2-19767] -
PEDF in the spiral ganglion following furosemide injection. (A–F) PEDF protein staining of guinea pig spiral ganglion following IV furosemide injection. (A) In the control was PEDF localized in the cytoplasm of the spiral ganglion type I neurons. (B–E) The staining intensity after furosemide at the different intervals. (F) Negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35392272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunohistochemistry: Serpin F1/PEDF Antibody - BSA Free [NBP2-19767] -
PEDF in the spiral ganglion following furosemide injection. (A–F) PEDF protein staining of guinea pig spiral ganglion following IV furosemide injection. (A) In the control was PEDF localized in the cytoplasm of the spiral ganglion type I neurons. (B–E) The staining intensity after furosemide at the different intervals. (F) Negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35392272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunohistochemistry: Serpin F1/PEDF Antibody - BSA Free [NBP2-19767] -
PEDF in the spiral ganglion following furosemide injection. (A–F) PEDF protein staining of guinea pig spiral ganglion following IV furosemide injection. (A) In the control was PEDF localized in the cytoplasm of the spiral ganglion type I neurons. (B–E) The staining intensity after furosemide at the different intervals. (F) Negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35392272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunohistochemistry: Serpin F1/PEDF Antibody - BSA Free [NBP2-19767] -
PEDF in the lateral cochlear wall. PEDF protein localization in the lateral cochlear wall. Stria vascularis (SV); Spiral prominence (SP); Marginal cell (MC); Basal cell (BC); type I fibrocytes (*); type II fibrocytes (**). (A) PEDF was localized to the spiral ligament, the stria vascularis and the spiral prominence. In the spiral ligament were type I and II fibrocytes PEDF positive. (B) Inset of higher magnification of marked area in (A). The distribution of PEDF in sub-cellular level of stria vascularis and spiral ligament. (C) Negative control of the immunohistochemistry. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35392272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Serpin F1/PEDF Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Serpin F1/PEDF
Long Name
Serpine F1/Pigment Epithelium-derived Factor
Alternate Names
EPC-1, PEDF
Gene Symbol
SERPINF1
UniProt
Additional Serpin F1/PEDF Products
Product Documents for Serpin F1/PEDF Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Serpin F1/PEDF Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Serpin F1/PEDF Antibody - BSA Free
Powered by Bioz
Customer Reviews for Serpin F1/PEDF Antibody - BSA Free
There are currently no reviews for this product. Be the first to review Serpin F1/PEDF Antibody - BSA Free and earn rewards!
Have you used Serpin F1/PEDF Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...