Simple Plex AAV3 Cartridge

Catalog #: SPCKB-OT-012247 Datasheet / COA / SDS

Simple Plex AAV3 assay kit contains cartridge, Sample Diluent SD19, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-OT-012247
ST01B-OT-012247

Ella Automated ELISA And Simple Plex AAV3 Assay

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All AAV3 Simple Plex Assays

Product Details

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex AAV3 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bioprocess Samples (25 uL)

Sensitivity

0.37 10^6 capsids/mL

Assay Range

3.5 - 5,400 10^6 capsids/mL (Bioprocess Samples)

Specificity

This assay recognizes intact AAV3 capsids.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of Adeno-Associated Virus type 3 (AAV3) intact capsids in Bioprocess samples in Sample Diluent SD19.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Bioprocess Samples

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	54
Mean (10⁶ capsids/mL)	6.4	161	7.48	171
Standard Deviation	0.3	4.6	0.51	11.6
CV%	4.5	2.9	6.8	6.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Bioprocess Samples (n=6)	97	94-99

Linearity

Samples containing and/or spiked with high concentrations of AAV3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex AAV3 Assay. Assay range is 3.54-5,400 10⁶ capsids/mL.