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Simple Plex AAV8 Cartridge

Catalog #: SPCKB-OT-007970 Datasheet / COA / SDS
Simple Plex AAV8 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD19, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-OT-007970
ST01B-OT-007970
AAV8 Simple Plex Assay
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex AAV8 Cartridge Summary

Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of Adeno-Associated Virus type 8 (AAV8) intact capsids in bioprocess samples.This assay uses PROGEN's AAV8 (ADK8) antibody.

Recovery

Recovery was not necessary as AAV8 shows natural linearity data from a neat sample to a 1:16 dilution. 

Linearity

Samples containing and/or spiked with high concentrations of AAV8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
AAV8 Simple Plex Assay Linearity

Scientific Data

N/A View Larger

AAV8 Simple Plex Assay Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

FAQs

  1. Do the AAV assays recognize fully-assembled capsids?

    • Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.

  2. Do the AAV assays recognize both natural and recombinant AAV?

    • Yes, the AAV assays detect both natural and recombinant AAV. 

  3. Does the AAV8 assay recognize other AAV serotypes?

    • This assay recognizes intact AAV8 capsids. The AAV8 antibody (ADK8) used in this assay cross-reacts with AAV1, AAV3, AAV7, and AAVrh10.

  4. Do the Simple Plex AAV assays distinguish between full and empty capsids?

    • No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.

  5. What diluent is provided with the AAV assays?

    • The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use. 

  6. What is the minimum required dilution (MRD) for bio-process samples?

    • All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery. 

View all Simple Plex Assays FAQs

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