Simple Plex AAV8 Cartridge
R&D Systems | Catalog # SPCKB-OT-007970
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
0.1 106 capsids/mL (Bioprocess Samples)
Assay Range
2.47-9428 106 capsids/mL (Bioprocess Samples)
Simple Plex AAV8 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Bioprocess Samples
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 47 | 45 |
| Mean (10<sup>6</sup> capsids/mL) | 23.1 | 1073 | 49.2 | 2202 |
| Standard Deviation | 0.658 | 55.4 | 4.51 | 99.3 |
| CV% | 2.9 | 5.2 | 9.2 | 4.5 |
Recovery for Simple Plex AAV8 Cartridge
Recovery was not necessary as AAV8 shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Samples containing and/or spiked with high concentrations of AAV8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex AAV8 Cartridge
AAV8 Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: AAV8
Long Name
Additional AAV8 Products
Product Documents for Simple Plex AAV8 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex AAV8 Cartridge
For research use only
Citations for Simple Plex AAV8 Cartridge
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FAQs for Simple Plex AAV8 Cartridge
-
Q: Do the AAV assays recognize both natural and recombinant AAV?
A: Yes, the AAV assays detect both natural and recombinant AAV.
-
Q: Do the AAV assays recognize fully-assembled capsids?
A: Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.
-
Q: Do the Simple Plex AAV assays distinguish between full and empty capsids?
A: No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.
-
Q: Does the AAV8 assay recognize other AAV serotypes?
A: This assay recognizes intact AAV8 capsids. The AAV8 antibody (ADK8) used in this assay cross-reacts with AAV1, AAV3, AAV7, and AAVrh10.
-
Q: What diluent is provided with the AAV assays?
A: The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.
-
Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.
-
Q: Do the AAV assays recognize both natural and recombinant AAV?
A: Yes, the AAV assays detect both natural and recombinant AAV.
-
Q: Do the AAV assays recognize fully-assembled capsids?
A: Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.
-
Q: Do the Simple Plex AAV assays distinguish between full and empty capsids?
A: No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.
-
Q: Does the AAV8 assay recognize other AAV serotypes?
A: This assay recognizes intact AAV8 capsids. The AAV8 antibody (ADK8) used in this assay cross-reacts with AAV1, AAV3, AAV7, and AAVrh10.
-
Q: What diluent is provided with the AAV assays?
A: The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.
-
Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.
-
Q: Do the AAV assays recognize both natural and recombinant AAV?
A: Yes, the AAV assays detect both natural and recombinant AAV.
-
Q: Do the AAV assays recognize fully-assembled capsids?
A: Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.
-
Q: Do the Simple Plex AAV assays distinguish between full and empty capsids?
A: No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.
-
Q: Does the AAV8 assay recognize other AAV serotypes?
A: This assay recognizes intact AAV8 capsids. The AAV8 antibody (ADK8) used in this assay cross-reacts with AAV1, AAV3, AAV7, and AAVrh10.
-
Q: What diluent is provided with the AAV assays?
A: The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.
-
Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.
-
Q: Do the AAV assays recognize both natural and recombinant AAV?
A: Yes, the AAV assays detect both natural and recombinant AAV.
-
Q: Do the AAV assays recognize fully-assembled capsids?
A: Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.
-
Q: Do the Simple Plex AAV assays distinguish between full and empty capsids?
A: No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.
-
Q: Does the AAV8 assay recognize other AAV serotypes?
A: This assay recognizes intact AAV8 capsids. The AAV8 antibody (ADK8) used in this assay cross-reacts with AAV1, AAV3, AAV7, and AAVrh10.
-
Q: What diluent is provided with the AAV assays?
A: The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.
-
Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.
-
Q: Do the AAV assays recognize both natural and recombinant AAV?
A: Yes, the AAV assays detect both natural and recombinant AAV.
-
Q: Do the AAV assays recognize fully-assembled capsids?
A: Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.
-
Q: Do the Simple Plex AAV assays distinguish between full and empty capsids?
A: No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.
-
Q: Does the AAV8 assay recognize other AAV serotypes?
A: This assay recognizes intact AAV8 capsids. The AAV8 antibody (ADK8) used in this assay cross-reacts with AAV1, AAV3, AAV7, and AAVrh10.
-
Q: What diluent is provided with the AAV assays?
A: The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.
-
Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.
-
Q: Do the AAV assays recognize both natural and recombinant AAV?
A: Yes, the AAV assays detect both natural and recombinant AAV.
-
Q: Do the AAV assays recognize fully-assembled capsids?
A: Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.
-
Q: Do the Simple Plex AAV assays distinguish between full and empty capsids?
A: No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.
-
Q: Does the AAV8 assay recognize other AAV serotypes?
A: This assay recognizes intact AAV8 capsids. The AAV8 antibody (ADK8) used in this assay cross-reacts with AAV1, AAV3, AAV7, and AAVrh10.
-
Q: What diluent is provided with the AAV assays?
A: The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.
-
Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.