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Simple Plex Cell Activation Panel 1

R&D Systems | Catalog # ST01C-CS-003222

Simple Plex 32x4 panel containing Granzyme B, IL-2, IFN-gamma 3rd Gen, and TNF-alpha 2nd Gen with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with CS (cell culture).
R&D Systems
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Key Product Details

Features

The Simple Plex Human Multianalyte Assay is for the detection of human GRANB, IFN gamma, IL-2, and TNF-alpha in Cell Culture Supernates.

 
Sample Type & Volume Required Per Well Cell Culture Supernates (25 uL)
Sensitivity GRANB 1.31 pg/mL; IFN gamma 0.17 pg/mL; IL-2 0.54 pg/mL; TNF-alpha 0.3 pg/mL
Assay Range GRANB 1.31-5,000 pg/mL; IFN gamma 0.17-4,000 pg/mL; Il-2 0.54-2,050 pg/mL; TNF-alpha 0.3-1,160 pg/mL

Species

Human

Applications

Simple Plex Automated Immunoassay

Product Summary for Simple Plex Cell Activation Panel 1

Product Specifications

Reactivity Human
Specificity Natural and recombinant human GRANB, IFN gamma, IL-2 and TNF alpha.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
GRANB n 16 16 16 16
Mean (pg/mL) 28 1357 28.7 1377
Standard Deviation 1.26 47.1 2.87 143
CV (%) 4.5 3.5 10 10.4
IFN gamma 3rd gen n 16 16 16 16
Mean (pg/mL) 21.1 1175 23.4 1192
Standard Deviation 1.48 33.4 2.52 99.4
CV (%) 7 2.8 10.8 8.3
IL-2 n 16 16 16 16
Mean (pg/mL) 15 648 13 608
Standard Deviation 0.8 32.7 1.13 34
CV (%) 5.3 5.1 8.7 5.6
TNF alpha 2nd Gen n 16 16 16 16
Mean (pg/mL) 6.99 383 0.662 36.9
Standard Deviation 0.345 20.6 7.81 408
CV (%) 4.9 5.4 8.5 9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

  Sample Type Average % Recovery Range %
GRANB EDTA Plasma (n=4) 103 91-117
Heparin Plasma (n=4) 95 86-107
Serum (n=4) 105 91-119
IFN gamma EDTA Plasma (n=4) 89 88-90
Heparin Plasma (n=4) 90 83-99
Serum (n=4) 95 83-102
IL-2 EDTA Plasma (n=4) 86 69-1002
Heparin Plasma (n=4) 90 70-103
Serum (n=4) 92 69-102
TNF alpha 2nd Gen EDTA Plasma (n=4) 97 89-105
Heparin Plasma (n=4) 89 77-96
Serum (n=4) 99 92-108
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Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

Additional Multiplex Cytokine Assay Kits Products

Product Documents for Simple Plex Cell Activation Panel 1

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Cell Activation Panel 1

For research use only

Citations for Simple Plex Cell Activation Panel 1

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