Simple Plex Cell Supernatant 1 Panel

R&D Systems | Catalog # ST01C-CS-005504

Simple Plex 32x4 panel containing IL-12 p70, IL-15, IL-18/IL-1F4, and IL-2 with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with CS (cell culture).
R&D Systems
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Key Product Details

Features

The Simple Plex Human Multianalyte Assay is for the detection of human IL-12 p70, IL-15, IL-18/IL-1F4, and IL-2 in cell culture supernatant samples.

 
Sample Type & Volume Required Per Well Cell Culture Supernatant (25 uL)
Sensitivity IL-12 p70 0.39 pg/mL; IL-15 0.19 pg/mL; IL-18/IL-1F4 0.2 pg/mL; IL-2 0.18 pg/mL
Assay Range IL-12 p70 0.62-5,890 pg/mL; IL-15 0.51 - 1,950 pg/mL; IL-18/IL-1F4 2.4 - 3,660 pg/mL; IL-2 0.54 - 2,050 pg/mL

Species

Human

Applications

Simple Plex Automated Immunoassay

Product Summary for Simple Plex Cell Supernatant 1 Panel

Product Specifications

Reactivity Human
Specificity Natural and recombinant human IL-12 p70, IL-15, IL-18/IL-1F4, and IL-2.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
IL-12 p70 n 16 16 16 16
Mean (pg/mL) 36.2 1657 33.2 1587
Standard Deviation 1.25 90 1.95 137
CV (%) 3.4 5.4 5.9 8.6
IL-15 n 16 16 16 16
Mean (pg/mL) 10.3 540 10.8 562
Standard Deviation 0.666 29.6 0.598 40.2
CV (%) 6.5 5.5 5.6 7.2
IL-18/IL-1F4 n 16 16 16 16
Mean (pg/mL) 10.5 569 10.3 587
Standard Deviation 0.524 18.7 0.882 58.4
CV (%) 5.2 3.3 8.6 10
IL-2 n 16 16 16 16
Mean (pg/mL) 15 648 13 608
Standard Deviation 0.8 32.7 1.13 34
CV (%) 5.3 5.1 8.7 5.6
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Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

Additional Multiplex Cytokine Assay Kits Products

Product Documents for Simple Plex Cell Supernatant 1 Panel

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Cell Supernatant 1 Panel

For research use only

Citations for Simple Plex Cell Supernatant 1 Panel

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