Simple Plex CHO HCP 3G-1 Cartridge

Catalog #: SPCKB-OT-003714 Datasheet / COA / SDS
Simple Plex CHO HCP 3G-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD19, and wash buffer
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Product Details

Simple Plex CHO HCP 3G-1 Cartridge Summary

Sample Type & Volume Required Per Well*
Bioprocess Samples (25 uL)
0.26 ng/mL
Assay Range
0.3 - 403 ng/mL (Bioprocess Samples)

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of Chinese Hamster Ovary Host Cell Protein (CHO HCP) in bioprocess samples. 


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (ng/mL) 3.12 3.42 158 162
Standard Deviation 0.217 0.343 14 23.4
CV% 6.9 10 8.9 14.4


Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
High (n=4) 110 104-116
Low (n=4) 96 76-111
Mid (n=4) 101 92-110


Samples containing and/or spiked with high concentrations of CHO HCP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


  1. What is the minimum required dilution (MRD) for bio-process samples?

    • All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery. 

View all Simple Plex Assays FAQs

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