Simple Plex CHO HCP 3G-1 Cartridge
R&D Systems | Catalog # SPCKB-OT-003714
In Partnership with Cygnus Technologies
The Simple Plex CHO HCP 3G-1 assay (SPCKB-OT-003714) on Ella is based on the same antibodies currently used in the Cygnus CHO HCP ELISA Kit, 3G (F550-1).
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex CHO HCP 3G-1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Bioprocess Samples
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 44 | 43 |
| Mean (ng/mL) | 3.12 | 158 | 3.42 | 162 |
| Standard Deviation | 0.217 | 14.0 | 0.343 | 23.4 |
| CV% | 6.9 | 8.9 | 10.0 | 14.4 |
Recovery for Simple Plex CHO HCP 3G-1 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| High (n=4) | 110 | 104-116 |
| Low (n=4) | 96 | 76-111 |
| Mid (n=4) | 101 | 92-110 |
Linearity
Samples containing and/or spiked with high concentrations of CHO HCP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex CHO HCP 3G-1 Cartridge
Simple Plex CHO HCP 3G-1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex CHO HCP 3G-1 Assay. Assay range is 0.26-403 ng/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Host Cell Protein/HCP
Long Name
Additional Host Cell Protein/HCP Products
Product Documents for Simple Plex CHO HCP 3G-1 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex CHO HCP 3G-1 Cartridge
For research use only
Citations for Simple Plex CHO HCP 3G-1 Cartridge
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FAQs for Simple Plex CHO HCP 3G-1 Cartridge
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Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.