Simple Plex Cytokine Storm Panel

R&D Systems | Catalog # SPCKA-PS-009470

Simple Plex 16x4 panel containing IL-1 beta / IL-1F2, IL-6 3rd Gen, IL-8/CXCL8, and TNF-alpha 2nd Gen with sample diluent SD13. Part # starting with ST01 ships in 3-5 days. Sample type indicated with PS (Plasma/Serum).
R&D Systems
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Key Product Details

Features

The Simple Plex Human Multianalyte Assay is for the detection of human IL-1 beta, IL-6 3rd gen, IL-8, and TNF-alpha in serum and plasma (EDTA/Heparin).

 
Sample Type & Volume Required Per Well Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity IL-1 beta 0.16 pg/mL; IL-6 3rd gen 0.11 pg/mL; IL-8 0.19 pg/mL; TNF-alpha 0.3 pg/mL
Assay Range IL-1 beta 0.16-1,530 pg/mL; IL-6 3rd gen 0.28-2,652 pg/mL; IL-8 0.19-1,804 pg/mL; TNF-alpha 0.3-1,160 pg/mL

Species

Human

Product Summary for Simple Plex Cytokine Storm Panel

Product Specifications

Reactivity Human
Specificity Natural and recombinant human IL-1 beta/IL-1F2, IL-6 3rd gen, IL-8/CXCL8, and TNF alpha.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
IL-1 beta/IL-1F2 n 16 16 16 16
Mean (pg/mL) 8.66 446 8.42 410
Standard Deviation 0.427 16.5 0.342 23.3
CV (%) 4.9 3.7 4 5.7
IL-6 3rd gen n 16 16 40 40
Mean (pg/mL) 23.1 876 23.6 1008
Standard Deviation 0.899 72.1 1.82 77.7
CV (%) 3.9 8.2 7.7 7.7
IL-8/CXCL8 n 16 16 16 16
Mean (pg/mL) 28.7 1362 27.3 1463
Standard Deviation 1.41 89.8 1.41 125
CV (%) 4.9 6.6 5.2 8.6
TNF alpha 2nd Gen n 16 16 16 16
Mean (pg/mL) 6.99 383 0.662 36.9
Standard Deviation 0.345 20.6 7.81 408
CV (%) 4.9 5.4 8.5 9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

  Sample Type Average % Recovery Range %
IL-1 beta/IL-1F2 EDTA Plasma (n=4) 106 97-120
Heparin Plasma (n=4) 99 99-112
Serum (n=4) 105 96-112
IL-6 3rd gen EDTA Plasma (n=4) 92 86-102
Heparin Plasma (n=4) 87 85-90
Serum (n=4) 94 90-99
CCM (n=2) 104 103-106
Saliva (n=4) 102 102-102
Urine (n=4) 98 93-104
IL-8/CXCL8 EDTA Plasma (n=5) 89 75-102
Heparin Plasma (n=5) 95 88-102
Serum (n=3) 92 88-97
TNF alpha 2nd Gen EDTA Plasma (n=4) 97 89-105
Heparin Plasma (n=4) 89 77-96
Serum (n=4) 99 92-108

Linearity

Samples containing and/or spiked with high concentrations of human IL-1 beta/IL-1F2, IL-6 2nd Gen, IL-8/CXCL8, and TNF alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the respective assays.

Il-1 Beta linearity graph
Il-1 Beta linearity graph
Il-1 Beta linearity graph
Il-1 Beta linearity graph

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Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

Additional Multiplex Cytokine Assay Kits Products

Product Documents for Simple Plex Cytokine Storm Panel

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Cytokine Storm Panel

For research use only

Citations for Simple Plex Cytokine Storm Panel

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