Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay

R&D Systems | Catalog # SPCKB-PS-012421

Simple Plex Human Galectin-3BP assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

4.37 pg/mL

Assay Range

20.1-30652 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay Summary

Simple Plex Discovery assay is for the detection of human Galectin-3 Binding Protein (Galectin-3BP) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Galectin-3BP/MAC-2BP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Galectin-3BP. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 23 23
Mean (pg/mL) 225 9833 230 10003
Standard Deviation 5.96 314 25.0 601
CV% 2.6 3.2 10.9 6.0

Recovery for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 96 94-99

Linearity

Samples containing and/or spiked with high concentrations of human Galectin-3BP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Galectin-3BP/MAC-2BP Ella Assay

Scientific Data Images for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay

Simple Plex Discovery Human Galectin-3BP/MAC-2BP Ella Assay

Simple Plex Discovery Human Galectin-3BP/MAC-2BP Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3BP/MAC-2BP

Galectin-3 binding protein, also known as MAC-2BP, M2BP, and 90K, is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily. Galectin-3BP binds Galectin-3 with high affinity, and additionally binds Galectins -1 and -7, several Collagen types, Fibronectin, beta 1 Integrins, and Nidogen. It is widely expressed in extracellular fluids and in pericellular areas of cell-rich tissues. Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers. It has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity. Galectin-3BP upregulation has also been correlated with tumor aggressiveness.

 

Long Name

Galectin-3 Binding Protein

Alternate Names

90K, Galectin3BP, LGALS3BP, MAC-2BP

Entrez Gene IDs

3959 (Human)

Gene Symbol

LGALS3BP

Additional Galectin-3BP/MAC-2BP Products

Product Documents for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay

For research use only

Citations for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay

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