Simple Plex Discovery Mouse Total MMP-9 Assay

R&D Systems | Catalog # SPCKB-MP-012215

Simple Plex Mouset Total MMP-9 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD09, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Cell Culture Supernates (10 µL), Mouse Serum (10 µL), Rat Serum (10 µL), Mouse EDTA Plasma (25 µL), Rat EDTA Plasma (10 µL), Mouse Heparin Plasma (25 µL), Rat Heparin Plasma (10 µL)

Sensitivity

8.99 pg/mL

Assay Range

27.2-41458 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma)

Simple Plex Discovery Mouse Total MMP-9 Assay Summary

Simple Plex Discovery assay is for the detection of mouse and rat natural, active, and pro-Matrix Metalloproteinase 9 (total MMP-9) in cell culture supernatant (CCS)*, serum, and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD09. *CCS is validated for mouse only. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all MMP-9 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Recombinant MMP-9 and natural, active, pro, and TIMP-1 complexed MMP-9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Mouse Cell Culture Supernates, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum, Rat EDTA Plasma, Rat Heparin Plasma, Rat Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 38 38
Mean (pg/mL) 323 14773 305 14016
Standard Deviation 10.3 577 26.1 1438
CV% 3.2 3.9 8.6 10.3

Recovery for Simple Plex Discovery Mouse Total MMP-9 Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 101 98-105

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Total MMP-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Mouse/Rat Total MMP-9 Ella Assay

Scientific Data Images for Simple Plex Discovery Mouse Total MMP-9 Assay

Simple Plex Discovery Mouse Total MMP-9 Ella Assay

Simple Plex Discovery Mouse Total MMP-9 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

MMP-9 (Gelatinase B) is a metalloproteinase that degrades multiple extracellular matrix components including Gelatin, Collagens III, IV, V, and XI, Elastin, Nidogen-1, and Vitronectin. It also cleaves a variety of chemokines and growth factors (e.g. IL-1 beta, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, Latent TGF-beta, membrane bound TNF-alpha, VEGF, and FGF basic), Amyloid beta peptide, Substance P, and Myelin Basic Protein. MMP-9 can trigger signaling through various transmembrane proteins or inhibit signaling by inducing their shedding from the cell surface (e.g. CD44, E-Cadherin, Integrins, ICAM-1, and CD25/IL-2 R alpha). The activity of MMP-9 is regulated by its association with the TIMP-1 protease inhibitor. Circulating levels of MMP-9 are elevated in many inflammatory disorders including intraluminal thrombus formation, atherosclerosis, Crohn’s disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for Simple Plex Discovery Mouse Total MMP-9 Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Mouse Total MMP-9 Assay

For research use only

Citations for Simple Plex Discovery Mouse Total MMP-9 Assay

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