Simple Plex Discovery Control for Human Pro-Collagen I a 1

R&D Systems | Catalog # 897973

Control for use with Human Pro-Collagen I a 1 Simple Plex Assay Cartridge Kit
R&D Systems

Key Product Details

Species

Human

Product Summary for Simple Plex Discovery Control for Human Pro-Collagen I a 1

INTENDED USE AND DESCRIPTION: For use a quantitative control for the determination of human Pro-Collagen I α 1 concentrations in biological fluids. Controls are supplied lyophilized. Please refer to the lot specific certificate of analysis and Simple Plex Lyophilized Quality Control product insert for additional information.

Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

• Store lyophilized controls at 2-8 °C. Lyophilized controls are stable for up to 12 months from date of receipt.
• Reconstituted controls are stable for up to 6 months from date of preparation when stored at ≤ -70 °C. Storage at -20° C is not recommended.
• Individual frozen aliquots of low and high controls are designed as single-use entities. Thaw directly before use and discard any remaining volume.

Background: Pro-Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Long Name

Pro-Collagen I alpha 1

Additional Pro-Collagen I alpha 1 Products

Product Documents for Simple Plex Discovery Control for Human Pro-Collagen I a 1

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Control for Human Pro-Collagen I a 1

For research use only

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