Simple Plex Discovery Human Angiopoietin-like Protein 7

R&D Systems | Catalog # SPCKB-PS-015352

Simple Plex Human ANGPTL7 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

10.7 pg/mL

Assay Range

57.3-14000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Angiopoietin-like Protein 7 Summary

Simple Plex Discovery assay is for the detection of human Angiopoietin-like Protein 7 (ANGPTL7) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Angiopoietin-like Protein 7/ANGPTL7 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human ANGPTL7.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one ANGPTL7 assay.

Inter-Assay Precision (Precision between assays) Replicates of each ANGPTL7 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human ANGPTL7.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 35 35
Mean (pg/mL) 121 5309 108 4984
Standard Deviation 4.22 195 10.6 369
CV% 3.5 3.7 9.8 7.4

Recovery for Simple Plex Discovery Human Angiopoietin-like Protein 7

Recovery for the human ANGPTL7 assays was evaluated at three different spiked concentrations of ANGPTL7 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 106 106-107
EDTA Plasma (n=2) 98 97-100
Heparin Plasma (n=2) 97 94-99
Serum (n=2) 94 94-94

Linearity

Samples containing and/or spiked with high concentrations of ANGPTL7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human ANGPTL7 Assay Linearity

Scientific Data Images for Simple Plex Discovery Human Angiopoietin-like Protein 7

Simple Plex Discovery Human ANGPTL7 Assay Standard Curve

Simple Plex Discovery Human ANGPTL7 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human ANGPTL7 Assay. Assay range is 57.3-14,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 7/ANGPTL7

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Long Name

Angiopoietin-like Protein 7

Alternate Names

ANGPTL7, AngX, CDT6

Entrez Gene IDs

10218 (Human); 654812 (Mouse); 102127960 (Cynomolgus Monkey); 714204 (Rhesus Macaque)

Gene Symbol

ANGPTL7

Additional Angiopoietin-like Protein 7/ANGPTL7 Products

Product Documents for Simple Plex Discovery Human Angiopoietin-like Protein 7

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human Angiopoietin-like Protein 7

For research use only

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