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Simple Plex Discovery Human Angiopoietin-like Protein 7

Catalog #: SPCKB-PS-015352 Datasheet / COA / SDS
Simple Plex Human ANGPTL7 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-015352
SPCKA-PS-015352
SPCKC-PS-015352
Simple Plex Discovery Human ANGPTL7 Assay Standard Curve
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Product Details
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Angiopoietin-like Protein 7 Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
10.7 pg/mL
Assay Range
57.3 - 14,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human ANGPTL7.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Angiopoietin-like Protein 7 (ANGPTL7) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one ANGPTL7 assay
Inter-Assay Precision (Precision between assays) Replicates of each ANGPTL7 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human ANGPTL7

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 35 35
Mean (pg/mL) 121 5309 108 4984
Standard Deviation 4.22 195 10.6 369
CV% 3.5 3.7 9.8 7.4

Recovery

Recovery for the human ANGPTL7 assays was evaluated at three different spiked concentrations of ANGPTL7 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 106 106-107
EDTA Plasma (n=2) 98 97-100
Heparin Plasma (n=2) 97 94-99
Serum (n=2) 94 94-94

Linearity

Samples containing and/or spiked with high concentrations of ANGPTL7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Simple Plex Discovery Human ANGPTL7 Assay Linearity

Scientific Data

N/A Simple Plex Discovery Human ANGPTL7 Assay Standard Curve View Larger

Simple Plex Discovery Human ANGPTL7 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human ANGPTL7 Assay. Assay range is 57.3-14,000 pg/mL.

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 7/ANGPTL7

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Long Name:
Angiopoietin-like Protein 7
Entrez Gene IDs:
10218 (Human); 654812 (Mouse); 102127960 (Cynomolgus Monkey); 714204 (Rhesus Macaque)
Alternate Names:
angiopoietin-like 7; Angiopoietin-like factor; Angiopoietin-like Protein 7; angiopoietin-related protein 7; ANGPTL7; AngX; CDT6; CDT6angiopoietin-like factor (CDT6); Cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2

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