Simple Plex Discovery Human Complement Factor D Assay

R&D Systems | Catalog # SPCKB-PS-012122

Simple Plex Human Complement Factor D assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

2.24 pg/mL

Assay Range

12-45680 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Complement Factor D Assay Summary

Simple Plex Discovery assay is for the detection of human Complement Factor D in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Complement Factor D/Adipsin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

icrofluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Complement Factor D. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Complement Factor D assay.

Inter-Assay Precision (Precision between assays) Replicates of each Complement Factor D control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Complement Factor D.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 33 33
Mean (pg/mL) 355 18708 352 17948
Standard Deviation 25.0 847 40.8 1600
CV% 7.0 4.5 11.6 8.9

Recovery for Simple Plex Discovery Human Complement Factor D Assay

Recovery for the human Complement Factor D assays was evaluated at three different spiked concentrations of Complement Factor D protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 111 111-112

Linearity

Samples containing and/or spiked with high concentrations of Complement Factor D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Complement Factor D Assay Linearity

Scientific Data Images for Simple Plex Discovery Human Complement Factor D Assay

Simple Plex Discovery Human Complement Factor D Assay Standard Curve

Simple Plex Discovery Human Complement Factor D Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Complement Factor D Assay. Assay range is 12.0-45,680 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Complement Factor D/Adipsin

Complement Factor D, also known as adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3.3 Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors.

Alternate Names

Adipsin, ADN, AMBP-1, CFD, PFD

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Gene Symbol

CFD

Additional Complement Factor D/Adipsin Products

Product Documents for Simple Plex Discovery Human Complement Factor D Assay

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Product Specific Notices for Simple Plex Discovery Human Complement Factor D Assay

For research use only

Related Research Areas

Complement Pathway

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