Simple Plex Discovery Human Complement Factor D Assay New

Catalog #: SPCKB-PS-012122 Datasheet / COA / SDS

Simple Plex Human Complement Factor D assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-012122
SPCKA-PS-012122
SPCKC-PS-012122

Control Products Available

897164: Simple Plex Discovery Control for Human Complement Factor D

Simple Plex Discovery Human Complement Factor D Assay Standard Curve

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All Complement Factor D/Adipsin Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Complement Factor D Assay Summary

Assay Type

Automated sandwich ELISA

Format

icrofluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

2.24 pg/mL

Assay Range

12.0 - 45,680 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Complement Factor D.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Complement Factor D in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Complement Factor D assay

Inter-Assay Precision (Precision between assays) Replicates of each Complement Factor D control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Complement Factor D

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	33	33
Mean (pg/mL)	355	18708	352	17948
Standard Deviation	25	847	40.8	1600
CV%	7	4.5	11.6	8.9

Recovery

Recovery for the human Complement Factor D assays was evaluated at three different spiked concentrations of Complement Factor D protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	111	111-112

Linearity

Samples containing and/or spiked with high concentrations of Complement Factor D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human Complement Factor D Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Complement Factor D Assay. Assay range is 12.0-45,680 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Complement Factor D/Adipsin

Complement Factor D, also known as adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3.3 Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors.

Entrez Gene IDs:

1675 (Human); 11537 (Mouse); 54249 (Rat)

Alternate Names:

Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN