Simple Plex Discovery Human Galectin-1 Assay

R&D Systems | Catalog # SPCKB-PS-012160

Simple Plex Human Galectin-1 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

2.53 pg/mL

Assay Range

6.36-60700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Galectin-1 Assay Summary

Simple Plex Discovery assay is for the detection of human Galectin-1 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Galectin-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Galectin-1. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 22 22
Mean (pg/mL) 529 24878 429 20905
Standard Deviation 16.7 1174 35.2 1473
CV% 3.2 4.7 8.2 7.0

Recovery for Simple Plex Discovery Human Galectin-1 Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 108 108-109

Linearity

Samples containing and/or spiked with high concentrations of human Galectin-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Galectin-1 Ella Assay

Scientific Data Images for Simple Plex Discovery Human Galectin-1 Assay

Simple Plex Discovery Human Galectin-1 Ella Assay

Simple Plex Discovery Human Galectin-1 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-1

Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds Laminin, Fibronectin, Galectin-3BP/MAC-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity.

 

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Entrez Gene IDs

3956 (Human); 16852 (Mouse)

Gene Symbol

LGALS1

Additional Galectin-1 Products

Product Documents for Simple Plex Discovery Human Galectin-1 Assay

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human Galectin-1 Assay

For research use only

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