Simple Plex Discovery Human Galectin-9 Assay

R&D Systems | Catalog # SPCKB-PS-011825

Simple Plex Human Galectin-9 assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

3.71 pg/mL

Assay Range

20.2-12352 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human Galectin-9 Assay Summary

Simple Plex Discovery assay is for the detection of human Galectin-9 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Galectin-9 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Galectin-9. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 33 33
Mean (pg/mL) 87.8 4158 95.7 4456
Standard Deviation 9.74 126 9.79 299
CV% 8.6 3.0 10.2 6.7

Recovery for Simple Plex Discovery Human Galectin-9 Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 104 104-105

Linearity

Samples containing and/or spiked with high concentrations of recombinant human Galectin-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Galectin-9 Ella Assay

Scientific Data Images for Simple Plex Discovery Human Galectin-9 Assay

Simple Plex Discovery Galectin-9 Ella Assay

Simple Plex Discovery Galectin-9 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-9

Galectin-9 is a secreted carbohydrate-binding protein with two lectin domains connected by a linker region. Progressive deletion within the linker region generates an isoform known as Ecalectin or UAT. Galectin-9 binds to carbohydrate moieties of IgE, preventing immune complex formation, mast cell degranulation, and asthmatic and cutaneous anaphylaxis reactions. It functions as an eosinophil chemoattractant and induces the maturation of dendritic cells which promote Th1 polarization. Galectin-9 binds to TIM-3, inhibiting Th1 cell and CD8+ T cell responses but promoting Treg differentiation and activity. Galectin-9 suppresses tumor cell metastasis by interfering with the associations between hyaluronic acid and CD44 and between VCAM-1 and Integrin alpha4 beta1. The Ecalectin isoform (UAT; urate transporter) can be expressed as an integral membrane protein and mediate the cellular efflux of urate.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Entrez Gene IDs

3965 (Human); 16859 (Mouse)

Gene Symbol

LGALS9

Additional Galectin-9 Products

Product Documents for Simple Plex Discovery Human Galectin-9 Assay

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human Galectin-9 Assay

For research use only

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