Simple Plex Discovery Human NF-H Assay

R&D Systems | Catalog # SPCKB-PS-013074

Simple Plex Discovery Human NF-H Assay kit for use on the Ella instrument. Contains Cartridge, Sample Diluent SD06, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

2.87 pg/mL

Assay Range

7.46-28480 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Discovery Human NF-H Assay Summary

Simple Plex Discovery assay is for the detection of human Neurofilament Heavy (NF-H) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD06.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all NF-H Simple Plex »

Compatible Instruments

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Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human NF-H.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 51 51
Mean (pg/mL) 48.5 2345 60.8 2645
Standard Deviation 1.31 70.0 8.55 274
CV% 2.7 3.0 14.1 10.3

Recovery for Simple Plex Discovery Human NF-H Assay

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 99 95-103
EDTA Plasma (n=4) 94 83-108
Heparin Plasma (n=4) 103 98-107
Serum (n=4) 102 82-115

Linearity

Samples containing and/or spiked with high concentrations of human NF-H were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human NF-H Ella Assay

Scientific Data Images for Simple Plex Discovery Human NF-H Assay

Simple Plex Discovery Human NF-H Ella Assay

Simple Plex Discovery Human NF-H Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NF-H

Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Entrez Gene IDs

4744 (Human)

Gene Symbol

NEFH

Additional NF-H Products

Product Documents for Simple Plex Discovery Human NF-H Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human NF-H Assay

For research use only

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