Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

R&D Systems | Catalog # SPCKB-PS-014623

Simple Plex Human TRANCE assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

3.1 pg/mL

Assay Range

21-32000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay Summary

Simple Plex Discovery assay is for the detection of human TNF-Related Activin-induced Cytokine (TRANCE) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TRANCE/TNFSF11/RANK L Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TRANCE.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TRANCE assay.

Inter-Assay Precision (Precision between assays) Replicates of each TRANCE control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TRANCE.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 24 24
Mean (pg/mL) 198 9796 253 12325
Standard Deviation 8.65 482 28.1 1286
CV% 4.4 4.9 11.1 10.4

Recovery for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

Recovery for the human TRANCE assays was evaluated at three different spiked concentrations of TRANCE protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 112 107-117
EDTA Plasma (n=2) 98 81-116
Heparin Plasma (n=2) 114 109-120
Serum (n=2) 108 101-115

Linearity

Samples containing and/or spiked with high concentrations of human TRANCE were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human TRANCE Assay Linearity

Scientific Data Images for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

Simple Plex Human TRANCE Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TRANCE Assay. Assay range is 21.0-32,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TRANCE/TNFSF11/RANK L

TRANCE (TNF-related activation-induced cytokines), also known as RANK Ligand, OPGL, or ODF, is a TNF superfamily ligand produced by osteoblasts, bone marrow stromal cells, and activated T cells. It signals through RANK and is antagonized by the decoy receptor Osteoprotegerin. TRANCE stimulates bone resorption, is required for osteoclast differentiation, and is associated with the development of osteoporosis, vascular calcification, osteopetrosis, and progestin-induced breast cancer. It also mediates body temperature regulation and is essential for the development of a lactating mammary gland.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

For research use only

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