Simple Plex Discovery Human Uromodulin Assay

R&D Systems | Catalog # SPCKB-PS-014653

Simple Plex Human Uromodulin assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

1.34 pg/mL

Assay Range

16.8-64000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Discovery Human Uromodulin Assay Summary

Simple Plex Discovery assay is for the detection of human Uromodulin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Uromodulin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Uromodulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Uromodulin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Uromodulin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Uromodulin.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 36 36
Mean (pg/mL) 487 24539 493 23178
Standard Deviation 9.33 870 31.1 1206
CV% 1.9 3.5 6.3 5.2

Recovery for Simple Plex Discovery Human Uromodulin Assay

Recovery for the human Uromodulin assays was evaluated at three different spiked concentrations of Uromodulin protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 96 92-101

Linearity

Samples containing and/or spiked with high concentrations of Uromodulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human Uromodulin Assay Linearity

Scientific Data Images for Simple Plex Discovery Human Uromodulin Assay

Simple Plex Discovery Human Uromodulin Assay Standard Curve

Simple Plex Discovery Human Uromodulin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Uromodulin Assay. Assay range is 16.8-64,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Uromodulin

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85 - 95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-α and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Human Uromodulin is GPI-linked. Human Uromodulin is 78% aa identical to mouse Uromodulin.

Alternate Names

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, Uromucoid

Entrez Gene IDs

7369 (Human); 22242 (Mouse); 25128 (Rat)

Gene Symbol

UMOD

Additional Uromodulin Products

Product Documents for Simple Plex Discovery Human Uromodulin Assay

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Discovery Human Uromodulin Assay

For research use only

Citations for Simple Plex Discovery Human Uromodulin Assay

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