Simple Plex Discovery Human Uromodulin Assay New

Catalog #: SPCKB-PS-014653 Datasheet / COA / SDS

Simple Plex Human Uromodulin assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer

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SPCKB-PS-014653
SPCKA-PS-014653
SPCKC-PS-014653

Control Products Available

897694: Simple Plex Discovery Control for Human Uromodulin

Simple Plex Discovery Human Uromodulin Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human Uromodulin Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)

Sensitivity

1.34 pg/mL

Assay Range

16.8 - 64,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human Uromodulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Uromodulin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Uromodulin assay

Inter-Assay Precision (Precision between assays) Replicates of each Uromodulin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Uromodulin

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	36	36
Mean (pg/mL)	487	24539	493	23178
Standard Deviation	9.33	870	31.1	1206
CV%	1.9	3.5	6.3	5.2

Recovery

Recovery for the human Uromodulin assays was evaluated at three different spiked concentrations of Uromodulin protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	96	92-101

Linearity

Samples containing and/or spiked with high concentrations of Uromodulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human Uromodulin Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Uromodulin Assay. Assay range is 16.8-64,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Uromodulin

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85 - 95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-α and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Human Uromodulin is GPI-linked. Human Uromodulin is 78% aa identical to mouse Uromodulin.

Entrez Gene IDs:

7369 (Human); 22242 (Mouse); 25128 (Rat)

Alternate Names:

ADMCKD2; FJHN; HNFJ; HNFJ1; MCKD2; Tamm-Horsfall glycoprotein; Tamm-Horsfall urinary glycoprotein; THGP; THP; UMOD; uromodulin (uromucoid, Tamm-Horsfall glycoprotein); Uromodulin; Uromucoid