Simple Plex Discovery Rat MMP-9 Assay New

Catalog #: SPCKB-RP-014262 Datasheet / COA / SDS

Simple Plex Rat MMP-9 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD09, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-RP-014262
SPCKA-RP-014262
SPCKC-RP-014262

Control Products Available

897082: Simple Plex Discovery Control for Mouse/Rat MMP-9

Simple Plex Discovery Rat MMP-9 Uteroglobin Ella Assay

3 Images

Product Details

Supplemental Products

Reviews

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Rat MMP-9 Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL)

Sensitivity

8.99 pg/mL

Assay Range

27.2 - 41,458 pg/mL (Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Specificity

Recombinant MMP-9 and natural, active, pro, and TIMP-1 complexed MMP-9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of rat natural, active, and pro-Matrix Metalloproteinase 9 (total MMP-9) in serum, and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD09.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	38
Mean (pg/mL)	323	14773	305	14016
Standard Deviation	10.3	577	26.1	1438
CV%	3.2	3.9	8.6	10.3

Recovery

Recovery was not necessary as rat MMP-9 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of rat Total MMP-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

View Larger

Simple Plex Discovery Rat MMP-9 Uteroglobin Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:

Matrix Metalloproteinase 9

Entrez Gene IDs:

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Alternate Names:

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase