Simple Plex Endonuclease Cartridge
R&D Systems | Catalog # SPCKC-OT-008612
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Endonuclease Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Bioprocess Samples
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 40 | 43 |
| Mean (pg/mL) | 191 | 5922 | 126 | 5636 |
| Standard Deviation | 12.2 | 194 | 14.6 | 576 |
| CV% | 6.4 | 3.3 | 11.6 | 10.2 |
Recovery for Simple Plex Endonuclease Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Bioprocess Samples (n=3) | 94 | 90-97 |
Linearity
Samples containing and/or spiked with high concentrations Endonuclease were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Endonuclease Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Endonuclease Assay. Assay range is 1.97-18,750 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Product Documents for Simple Plex Endonuclease Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Endonuclease Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Endonuclease Cartridge
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FAQs for Simple Plex Endonuclease Cartridge
-
Q: Does the Simple Plex Endonuclease assay detect Denarase® and Benzonase®?
A: Yes, both Denarase® and Benzonase® are detectable in this assay.
-
Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.
-
Q: Does the Simple Plex Endonuclease assay detect Denarase® and Benzonase®?
A: Yes, both Denarase® and Benzonase® are detectable in this assay.
-
Q: What is the minimum required dilution (MRD) for bio-process samples?
A: All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.