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Simple Plex Endonuclease Cartridge

Catalog #: SPCKC-OT-008612 Datasheet / COA / SDS
Simple Plex Endonuclease assay kit for use on Ella instrument. Contains cartridge, sample diluent SD19, and wash buffer
Catalog # Availability Size / Price Qty
SPCKA-OT-008612
SPCKC-OT-008612
ST01C-OT-008612
Control Products Available
896993: Simple Plex Control for Endonuclease
Endonuclease Ella Assay
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Endonuclease Cartridge Summary

Sample Type & Volume Required Per Well*
Bioprocess Samples (25 uL)
Sensitivity
0.415 pg/mL
Assay Range
2.0 - 18,750 pg/mL (Bioprocess Samples)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of Endonuclease in bioprocess samples. Denarase® and Benzonase® are detectable in this assay.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 40 43
Mean (pg/mL) 191 5922 126 5636
Standard Deviation 12.2 194 14.6 576
CV% 6.4 3.3 11.6 10.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Bioprocess Samples (n=3) 94 90-97

Linearity

Samples containing and/or spiked with high concentrations Endonuclease were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Endonuclease Ella Assay Linearity

Scientific Data

N/A Endonuclease Ella Assay Standard Curve View Larger

Endonuclease Ella Assay Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

FAQs

  1. Does the Simple Plex Endonuclease assay detect Denarase® and Benzonase®?

    • Yes, both Denarase® and Benzonase® are detectable in this assay.

  2. What is the minimum required dilution (MRD) for bio-process samples?

    • All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery. 

View all Simple Plex Assays FAQs

Simple Plex Assay Controls

Simple Plex Control for Endonuclease

896993

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