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Simple Plex HEK 293 HCP 3G Cartridge

Catalog #: SPCKB-OT-007066 Datasheet / COA / SDS
Simple Plex Host Cell Protein/HCP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD19, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-OT-007066
HEK HCP Simple Plex Assay
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Simple Plex HEK 293 HCP 3G Cartridge Summary

Sample Type & Volume Required Per Well*
Bioprocess Samples (25 uL)
Sensitivity
0.2 ng/mL
Assay Range
1.6 - 400 ng/mL (Bioprocess Samples)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of Human Embryonic Kidney Host Cell Protein (HEK 293 HCP 3G) in bioprocess samples.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 8 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 8 8 81 79
Mean (ng/mL) 3.16 147 3.36 141
Standard Deviation 0.38 7.3 0.48 14.9
CV% 12 5 14.3 10.6

Recovery

Recovery at two different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Bioprocess Samples (n=12) 111 100-123

Linearity

Samples containing and/or spiked with high concentrations of HEK 293 HCP 3G were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
HEK HCP Simple Plex Assay Linearity

Scientific Data

N/A View Larger

HEK HCP Simple Plex Assay

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

FAQs

  1. What is the minimum required dilution (MRD) for bio-process samples?

    • All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery. 

View all Simple Plex Assays FAQs

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