Simple Plex Human alpha 2-Macroglobulin Cartridge

R&D Systems | Catalog # SPCKB-PS-008932

Simple Plex Human alpha 2-Macroglobulin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

16.97 pg/mL

Assay Range

81.92-125000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid)

Simple Plex Human alpha 2-Macroglobulin Cartridge Summary

Simple Plex assay for the detection of human alpha 2-Macroglobulin ( alpha 2-Macroglobulin) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all alpha 2-Macroglobulin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human alpha 2-Macroglobulin. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 64 68
Mean (pg/mL) 515 40826 766 38297
Standard Deviation 29.7 3940 85.8 4381
CV% 5.8 9.7 11.2 11.4

Recovery for Simple Plex Human alpha 2-Macroglobulin Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 101 98-104

Linearity

Samples containing and/or spiked with high concentrations of human alpha 2-Macroglobulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human alpha 2-Macroglobulin Cartridge

Human alpha 2-Macroglobulin Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha 2-Macroglobulin

Alpha 2-Macroglobulin (A2M) is an abundant plasma glycoprotein that has structural similarity to other A2M family members including complement components C3, C4 and C5. A2M is a tetramer (720-kDa) of 4 identical subunits (180-kDa) formed by two disulfide bond-linked dimers. It is a general and irreversible protease inhibitor capable of inhibiting all four classes of proteases by a unique trapping mechanism. A2M also binds many cytokines and growth factors. In the brain, A2M binds soluble beta Amyloid. A2M is recognized as a late onset Alzheimers disease gene.

Alternate Names

A2M, alpha 2Macroglobulin, CPAMD5

Entrez Gene IDs

2 (Human)

Gene Symbol

A2M

Additional alpha 2-Macroglobulin Products

Product Documents for Simple Plex Human alpha 2-Macroglobulin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human alpha 2-Macroglobulin Cartridge

For research use only

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