Simple Plex Human alpha 2-Macroglobulin Cartridge

Catalog #: SPCKB-PS-008932 Datasheet / COA / SDS

Simple Plex Human alpha 2-Macroglobulin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-00893
SPCKC-PS-008932
SPCKA-PS-00893

Control Products Available

897116: Simple Plex Control for Human alpha 2-Macroglobulin

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All alpha 2-Macroglobulin Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human alpha 2-Macroglobulin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

16.97 pg/mL

Assay Range

81.9 - 125,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid)

Specificity

Natural and recombinant human alpha 2-Macroglobulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human alpha 2-Macroglobulin ( alpha 2-Macroglobulin) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay)

Inter-Assay Precision (Precision between assays)

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	64	68
Mean (pg/mL)	515	40826	766	38297
Standard Deviation	29.7	3940	85.8	4381
CV%	5.8	9.7	11.2	11.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	101	98-104

Linearity

Samples containing and/or spiked with high concentrations of human alpha 2-Macroglobulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Human alpha 2-Macroglobulin Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha 2-Macroglobulin

Alpha 2-Macroglobulin (A2M) is an abundant plasma glycoprotein that has structural similarity to other A2M family members including complement components C3, C4 and C5. A2M is a tetramer (720-kDa) of 4 identical subunits (180-kDa) formed by two disulfide bond-linked dimers. It is a general and irreversible protease inhibitor capable of inhibiting all four classes of proteases by a unique trapping mechanism. A2M also binds many cytokines and growth factors. In the brain, A2M binds soluble beta Amyloid. A2M is recognized as a late onset Alzheimers disease gene.

Entrez Gene IDs:

2 (Human)

Alternate Names:

A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; Alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7