Simple Plex Human Angiopoietin-2 Cartridge

Simple Plex Cartridge Kit for 72 samples, containing Angiopoietin-2 for use with Human Plasma/Serum. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

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Human Angiopoietin-2 Simple Plex Assay
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Simple Plex Human Angiopoietin-2 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity
3.96 pg/mL
Assay Range
4.0 - 15,124 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Angiopoietin-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Angiopoietin-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean 163 7021 155 7324
Standard Deviation 8.68 454 13.9 298
CV% 5.3 6.5 9 4.1

Recovery

Recovery was not necessary as Angiopoietin-2 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Angiopoietin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Angiopoietin-2 Simple Plex Assay Linearity

Data Examples

Human Angiopoietin-2 Simple Plex Assay

Human Angiopoietin-2 Simple Plex Assay Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine)
Alternate Names:
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand

FAQs

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