Simple Plex Human Angiopoietin-like Protein 4 Cartridge

R&D Systems | Catalog # SPCKB-PS-000291

Simple Plex Human Angiopoietin-like Protein 4/ANGPTL4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

93.2 pg/mL

Assay Range

546-333200 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Angiopoietin-like Protein 4 Cartridge Summary

Simple Plex assay is for the detection of human Angiopoietin-like 4 in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Angiopoietin-like Protein 4/ANGPTL4 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Angiopoietin-like 4.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Angiopoietin-like 4 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Angiopoietin-like 4 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Angiopoietin-like 4.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 19 18
Mean (pg/mL) 4324 190,343 5327 222,216
Standard Deviation 293 17,170 795 29,685
CV% 5.0 9.0 14.9 13.4

Recovery for Simple Plex Human Angiopoietin-like Protein 4 Cartridge

Recovery was not necessary as human Angiopoietin-like 4 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Angiopoietin-like 4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Angiopoietin-like 4 Assay Linearity

Scientific Data Images for Simple Plex Human Angiopoietin-like Protein 4 Cartridge

Simple Plex Human Angiopoietin-like 4 Assay Standard Curve

Simple Plex Human Angiopoietin-like 4 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Angiopoietin-like 4 Assay. Assay range is 546-333,200 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated. The coiled coil domain mediates the formation of variable sized disulfide-linked oligomers. This domain directly inhibits lipoprotein lipase (LPL), resulting in increased circulating triglyceride levels. ANGPTL4 circulates in association with HDL lipoproteins. Its expression in adipose tissue is induced by fasting and suppressed by feeding. In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells. The N-terminal fragment can function as an angiogenesis inhibitor. In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins alpha 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing. ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells.

Alternate Names

Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Gene Symbol

ANGPTL4

Additional Angiopoietin-like Protein 4/ANGPTL4 Products

Product Documents for Simple Plex Human Angiopoietin-like Protein 4 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Angiopoietin-like Protein 4 Cartridge

For research use only

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