Best Seller

Simple Plex Human BCMA/TNFRSF17 Cartridge

R&D Systems | Catalog # SPCKB-PS-000768

Simple Plex Human BCMA/TNFRSF17 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.54 pg/mL

Assay Range

2.62-10000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human BCMA/TNFRSF17 Cartridge Summary

Simple Plex assay is for the detection of human B Cell Maturation Antigen/TNFRSF17 (BCMA) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all BCMA/TNFRSF17 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human BCMA.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one BCMA assay.

Inter-Assay Precision (Precision between assays) Replicates of each BCMA control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BCMA.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 35 35
Mean (pg/mL) 58.8 2931 57.7 2971
Standard Deviation 2.96 170 6.40 283
CV% 5.0 5.8 11.1 9.5

Recovery for Simple Plex Human BCMA/TNFRSF17 Cartridge

Recovery for the human BCMA assays was evaluated at three different spiked concentrations of BCMA protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 111 108-113

Linearity

Samples containing and/or spiked with high concentrations of human BCMA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human BCMA Assay Linearity

Scientific Data Images for Simple Plex Human BCMA/TNFRSF17 Cartridge

Simple Plex Human BCMA Assay Standard Curve

Simple Plex Human BCMA Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human BCMA Assay. Assay range is 2.62-10,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BCMA/TNFRSF17

B cell maturation protein (BCMA) is a member of the TNF receptor superfamily (TNFRSF17). BCMA is expressed in immune organs and mature Bcell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. BCMA binding to APRIL or BAFF stimulates IgM production in peripheral blood B cells and increases the survival of cultured B cells. Mature human and mouse BCMA share 86% amino acid sequence identity.

Long Name

B Cell Maturation Factor

Alternate Names

CD269, TNFRSF13A, TNFRSF17

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Gene Symbol

TNFRSF17

Additional BCMA/TNFRSF17 Products

Product Documents for Simple Plex Human BCMA/TNFRSF17 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human BCMA/TNFRSF17 Cartridge

For research use only

Citations for Simple Plex Human BCMA/TNFRSF17 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human BCMA/TNFRSF17 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human BCMA/TNFRSF17 Cartridge and earn rewards!

Have you used Simple Plex Human BCMA/TNFRSF17 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card