Simple Plex Human BCMA/TNFRSF17 Cartridge

Catalog #: SPCKB-PS-000768 Datasheet / COA / SDS

Simple Plex Human BCMA/TNFRSF17 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000768
SPCKA-PS-000768
SPCKC-PS-000768
ST01B-PS-000768
ST01C-PS-000768

Control Products Available

898628: Simple Plex Control for Human BCMA/TNFRSF17

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Simple Plex Human BCMA Assay Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human BCMA/TNFRSF17 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.54 pg/mL

Assay Range

2.6 - 10,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human BCMA.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human B Cell Maturation Antigen/TNFRSF17 (BCMA) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one BCMA assay

Inter-Assay Precision (Precision between assays) Replicates of each BCMA control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BCMA

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	35	35
Mean (pg/mL)	58.8	2931	57.7	2971
Standard Deviation	2.96	170	6.4	283
CV%	5	5.8	11.1	9.5

Recovery

Recovery for the human BCMA assays was evaluated at three different spiked concentrations of BCMA protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	111	108-113

Linearity

Samples containing and/or spiked with high concentrations of human BCMA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human BCMA Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human BCMA Assay. Assay range is 2.62-10,000 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BCMA/TNFRSF17

B cell maturation protein (BCMA) is a member of the TNF receptor superfamily (TNFRSF17). BCMA is expressed in immune organs and mature Bcell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. BCMA binding to APRIL or BAFF stimulates IgM production in peripheral blood B cells and increases the survival of cultured B cells. Mature human and mouse BCMA share 86% amino acid sequence identity.

Long Name:

B Cell Maturation Factor

Entrez Gene IDs:

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names:

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17