Simple Plex Human beta-Synuclein Cartridge

R&D Systems | Catalog # SPCKB-PS-010127

Simple Plex Human beta-Synuclein assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

1.83 pg/mL

Assay Range

7.96-4856 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human beta-Synuclein Cartridge Summary

Simple Plex assay for the detection of human beta -Synuclein in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all beta-Synuclein Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant beta -Synuclein.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 41 40
Mean (pg/mL) 44.6 2168 44.3 2056
Standard Deviation 1.4 72.4 4.1 122
CV% 3.2 3.3 9.2 6.0

Recovery for Simple Plex Human beta-Synuclein Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 105 100-109
EDTA Plasma (n=4) 95 90-99
Heparin Plasma (n=4) 101 92-106
Serum (n=4) 102 98-107

Linearity

Samples containing and/or spiked with high concentrations of beta -Synuclein were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human beta-Synuclein Cartridge

Human beta-Synuclein Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta-Synuclein

The synuclein family includes synuclein-alpha, -beta, and -gamma, and synoretin. These small, highly conserved proteins are localized predominantly in the brain, concentrated in presynaptic nerve terminals. Synuclein-alpha is normally a soluble protein. Abnormal synuclein-alpha forms filamentous inclusions (Lewy bodies and Lewy neurites) in several neurodegenerative diseases, including Parkinson's disease (PD), dementia with Lewy bodies, and Alzheimer's disease. Synucleins beta and gamma (synoretin) associate with synuclein-alpha and inhibit its aggregation.

Alternate Names

SNCB, Synuclein-beta

Entrez Gene IDs

6620 (Human); 104069 (Mouse); 113893 (Rat)

Gene Symbol

SNCB

Additional beta-Synuclein Products

Product Documents for Simple Plex Human beta-Synuclein Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human beta-Synuclein Cartridge

For research use only

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