Simple Plex Human BMP-9 Cartridge

R&D Systems | Catalog # SPCKB-PS-000474

Simple Plex Human BMP-9 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.086 pg/mL

Assay Range

0.3-2900 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human BMP-9 Cartridge Summary

Simple Plex assay is for the detection of human Bone Morphogenic Protein 9 (BMP-9) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human BMP-9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one BMP-9 assay.

Inter-Assay Precision (Precision between assays) Replicates of each BMP-9 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BMP-9.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 23 23
Mean (pg/mL) 18.5 891 17.8 866
Standard Deviation 1.48 87.1 1.28 87.1
CV% 8.0 9.8 7.2 6.6

Recovery for Simple Plex Human BMP-9 Cartridge

Recovery for the human BMP-9 assays was evaluated at three different spiked concentrations of BMP-9 protein.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=6) 110 106-117

Linearity

Samples containing and/or spiked with high concentrations of human BMP-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human BMP-9 Assay Linearity

Scientific Data Images for Simple Plex Human BMP-9 Cartridge

Simple Plex Human BMP-9 Assay Standard Curve

Simple Plex Human BMP-9 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human BMP-9 Assay. Assay range is 0.3-2900 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BMP-9

 

BMP-9 (Bone morphogenetic protein 9, GDF-2) is expressed by non-parenchymal cells in the liver, where it promotes lipid metabolism and inhibits glucose production. BMP-9 exerts a prolonged hypoglycemic effect. BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype and induces the differentiation of mesenchymal stem cells into the chondrogenic lineage.

Long Name

Bone Morphogenetic Protein 9

Alternate Names

BMP9, GDF-2, GDF2

Entrez Gene IDs

2658 (Human); 12165 (Mouse)

Gene Symbol

GDF2

Additional BMP-9 Products

Product Documents for Simple Plex Human BMP-9 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human BMP-9 Cartridge

For research use only

Related Research Areas

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