Simple Plex Human C-Reactive Protein/CRP Cartridge

Simple Plex Cartridge Kit for 72 samples, containing CRP for use with Human Plasma / Serum. Includes wash buffer and sample diluent SD13. For use with the Ella automated immunoassay system

Ella: Your Next Generation ELISA

Ella's combination of performance and workflow brings your immunoassays to the next level. In just 90 minutes you get highly reproducible validated assay data with no manual steps. The assay performance behind that data includes sub-picogram level sensitivity, 4+ logs of dynamic range and reproducibility that rivals the best laboratory automation. All in a compact package that doesn't break your budget or your lab bench.
Human CRP Simple Plex Assay
3 Images
Product Details
FAQs
Supplemental Products
Reviews

Simple Plex Human C-Reactive Protein/CRP Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (25 nL), EDTA Plasma (25 nL), Heparin Plasma (25 nL)
Sensitivity
1.24 pg/mL
Assay Range
32.8 - 8,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human CRP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human CRP in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) <div>Each control was tested 16 times&nbsp;in one assay.</div>
Inter-Assay Precision (Precision between assays) <div>Replicates of each control were&nbsp;tested in multiple assays performed by at least three&nbsp;technicians using two lots of reagents.</div>

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean 291 18310 282 18318
Standard Deviation 19.6 783 14.2 1170
CV% 6.7 4.3 5 6.4

Recovery

Recovery was not necessary as CRP shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human CRP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Human CRP Simple Plex Linearity

Data Examples

Human CRP Simple Plex Assay

Human CRP Simple Plex Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Entrez Gene IDs:
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names:
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

FAQs

No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs

Automated ELISA Instruments

Reviews for Simple Plex Human C-Reactive Protein/CRP Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human C-Reactive Protein/CRP Cartridge and earn rewards!

Have you used Simple Plex Human C-Reactive Protein/CRP Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review