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Simple Plex Human C-Reactive Protein/CRP Cartridge

R&D Systems | Catalog # SPCKB-PS-000200

Simple Plex Human CRP assay kit contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

1.24 pg/mL

Assay Range

32.8-50000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human C-Reactive Protein/CRP Cartridge Summary

Simple Plex assay is for the detection of human C-Reactive Protein (CRP) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all C-Reactive Protein/CRP Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CRP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CRP assay.

Inter-Assay Precision (Precision between assays) Replicates of each CRP control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CRP.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 23 22
Mean (pg/mL) 291 18310 282 18318
Standard Deviation 19.6 783 14.2 1170
CV% 6.7 4.3 5.0 6.4

Recovery for Simple Plex Human C-Reactive Protein/CRP Cartridge

Recovery for the human CRP assays was evaluated at three different spiked concentrations of CD40 protein.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=3) 99 97-101

Linearity

Samples containing and/or spiked with high concentrations of human CRP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human CRP Assay Linearity

Scientific Data Images for Simple Plex Human C-Reactive Protein/CRP Cartridge

Simple Plex Human CRP Assay Standard Curve

Simple Plex Human CRP Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CRP Assay. Assay range is 32.8-50,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Alternate Names

CRP

Entrez Gene IDs

1401 (Human); 12944 (Mouse); 25419 (Rat)

Gene Symbol

CRP

Additional C-Reactive Protein/CRP Products

Product Documents for Simple Plex Human C-Reactive Protein/CRP Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human C-Reactive Protein/CRP Cartridge

For research use only

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