Simple Plex Human CD21 Cartridge
R&D Systems | Catalog # SPCKB-PS-000451
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CD21 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CD21 assay.
Inter-Assay Precision (Precision between assays) Replicates of each CD21 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CD21.
Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 15 | 15 |
| Mean (pg/mL) | 925 | 41,601 | 883 | 39,241 |
| Standard Deviation | 70.6 | 1969 | 44.7 | 1895 |
| CV% | 7.6 | 4.7 | 5.1 | 4.8 |
Recovery for Simple Plex Human CD21 Cartridge
Recovery was not necessary as human CD21 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human CD21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CD21 Cartridge
Simple Plex Human CD21 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CD21 Assay. Assay range is 91.8-140,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CD21
CD21, also known as complement receptor 2 (CR2), is a 145 kDa transmembrane protein that contains 15 tandem SCR/SUSHI repeats. CD21 is primarily expressed on B cells, follicular dendritic cells, and T cells. It binds the complement component fragments iC3b, C3d, and C3d,g and forms complexes with CD19 and CD35. CD21 is required for germinal center development, antibody production, and B cell tolerance. It also functions as a cellular uptake receptor for Epstein-Barr virus.
Alternate Names
Gene Symbol
Additional CD21 Products
Product Documents for Simple Plex Human CD21 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human CD21 Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human CD21 Cartridge
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