Simple Plex Human Chitinase 3-like 1 Cartridge
Simple Plex Human Chitinase 3-like 1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid
|Intra-Assay Precision||Inter-Assay Precision|
Recovery at three different spiked concentrations within the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=2)||80||75-84|
Human CHI3L1 Simple Plex Assay Standard Curve
Preparation and Storage
Background: Chitinase 3-like 1
Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.
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