Simple Plex Human Chitinase 3-like 1 Cartridge

Catalog #: SPCKB-PS-000255 Datasheet / COA / SDS
Simple Plex Human Chitinase 3-like 1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000255
SPCKA-PS-000255
SPCKC-PS-000255
Control Products Available
Human CHI3L1 Simple Plex Assay
3 Images
Product Details
FAQs
Supplemental Products
Reviews

Simple Plex Human Chitinase 3-like 1 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL), Urine (25 uL), Cerebrospinal Fluid (500 nL)
Sensitivity
6.68 pg/mL
Assay Range
6.7 - 25,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)
Specificity
Natural and recombinant human CHI3L1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Chitinase 3-like 1 (CHI3L1) in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 22 22
Mean (pg/mL) 341 30384 373 26287
Standard Deviation 23.5 1905 35.8 2724
CV% 6.9 6.3 9.6 10.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 80 75-84

Linearity

Samples containing and/or spiked with high concentrations of human CHI3L1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human CHI3L1 Simple Plex Assay Linearity

Data Examples

Human CHI3L1 Simple Plex Assay Standard Curve

Product Datasheets

You must select a language.

x

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chitinase 3-like 1

Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.

Entrez Gene IDs:
1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)
Alternate Names:
AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40

FAQs

No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs

Automated ELISA Instruments

Simple Plex Assay Controls

Reviews for Simple Plex Human Chitinase 3-like 1 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human Chitinase 3-like 1 Cartridge and earn rewards!

Have you used Simple Plex Human Chitinase 3-like 1 Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review